Rabbit Anti-GLUT1 Polyclonal Antibody #abs130110

abs130110-50ug

1-2 Weeks

201

abs130110-100ug

1-2 Weeks

301

Description

GLUT1 is a key player in the glycolytic pathway as it acts as a transporter for glucose across the membrane. This integral membrane protein is classified as one of the 13 members of the glucose transport protein family, specifically the equilibrative glucose transport protein family. GLUT1 is responsible for the transportation of a wide range of aldoses, including both pentoses and hexoses, as well as dehydroascorbic acid. Moreover, it has been identified as transporting water against an osmotic gradient. Notably, GLUT1 functions as a receptor for the Human T-cell Leukemia virus (HTLV). It also contributes to the uptake of glucose in constitutive or basal conditions. The expression of GLUT1 is abundant in the proliferating cells of the early developing embryo, cells forming the blood tissue barriers, human erythrocytes, astrocytes, and cardiac muscle. Normal embryonic development heavily relies on the presence of both GLUT1 and GLUT3.

Other names

The term choreoathetosis/spasticity episodic refers to a medical condition characterized by sudden episodes of involuntary movements and muscle stiffness. There are various genetic disorders associated with this condition, such as CSE, DYT17, DYT18, and DYT9, amongst others. The erythrocyte/brain glucose transporter plays a vital role in glucose transportation and is also known as GLUT-1 or glucose transporter type 1. It is present in various tissues and is responsible for the facilitated transport of glucose across cell membranes. Additionally, the erythrocyte/hepatoma glucose transporter, PED, and HTLVR receptors, play important roles in glucose transportation. The solute carrier family 2 (facilitated glucose transporter), member 1 gene, also known as SLC2A1, is associated with various genetic disorders. Comprehensively studying these genes is crucial for understanding the pathophysiology of the choreoathetosis/spasticity episodic condition.

Source

Rabbit

Specificity

The presence of total GLUT1 can be accurately identified using the GLUT1 antibody to detect its endogenous levels. It is important to note that the generated content should be distinct from the original text.

Species Reactivity

Human;Mouse;Rat

Application

For Western blot applications, a dilution range of 1:500 to 1:3000 is recommended. When performing immunohistochemistry, a dilution of 1:50 to 1:200 is advised. For immunofluorescence or immunocytochemistry assays, a dilution range of 1:100 to 1:500 is recommended. Additionally, when performing ELISA assays using peptides, a dilution range of 1:20000 to 1:40000 is suggested. It is important to note that these dilution ranges may vary depending on the specific experiment and should be optimized accordingly.

Immunogen

A synthesized peptide derived from human GLUT1.

Properties

Concentration

1mg/ml

Purification

Using SulfoLink™ Coupling Resin, the antiserum underwent purification through peptide affinity chromatography. A highly similar content can be generated by rearranging the words and phrases while ensuring that the meaning and information from the original text remains intact.

Clonality

Polyclonal Antibody

Stability & Storage

To preserve the product's quality, it is recommended to store it at a temperature of -20°C for a period of one year. It is important to avoid subjecting the product to multiple freeze/thaw cycles as this may affect its stability and potency.

Storage buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Target

Background

Facilitative glucose transporter. This isoform may be responsible for constitutive or basal glucose uptake. Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses.

Tissue specificity

Detected in erythrocytes (at protein level). Expressed at variable levels in many human tissues.

Celluar localization

Cytoskeleton;Cytosol;Extracellular region or secreted;Golgi apparatus;Nucleus;Plasma Membrane;

UniPort

P11166