Rabbit Anti-Factor VIII Polyclonal Antibody #abs124563

abs124563-50ul

In Stock

201

abs124563-100ul

In Stock

301

Other names

Factor VIII, also known as Antihemophilic factor or Coagulation factor VIII, is a protein that plays a critical role in blood clotting. It is encoded by the F8 gene and is located on the X chromosome. Deficiency or dysfunction of Factor VIII leads to Hemophilia A, a bleeding disorder that is inherited in an X-linked recessive pattern.
There are several isoforms of Factor VIII, including Coagulation factor VIII associated protein b, Coagulation factor VIII isoform b, and Coagulation factor VIII procoagulent component. Factor VIIIc, F8 protein, and FactorVIII are also used to refer to this protein.
In terms of its structure, Factor VIII has a heavy chain and a light chain that are linked by a metal ion. The heavy chain is further divided into two isoforms, a 200 kDa and a 92 kDa version. The B chain of Factor VIII is also important for its function.
Overall, Factor VIII is a crucial component of the blood clotting cascade and its deficiency can have serious medical consequences.

Source

Rabbit

Specificity

Factor VIII

Species Reactivity

Human;Mouse;Rat;Dog;Pig;Cow;Rabbit;

Application

ELISA: The recommended dilution for ELISA is 1:500-1000.
Immunohistochemistry - Paraffin (IHC-P): The appropriate dilution for IHC-P is 1:400-800.
Immunohistochemistry - Frozen (IHC-F): The recommended dilution for IHC-F is 1:400-800.
Immunofluorescence (IF): The suitable dilution for IF is 1:100-500, with antigen retrieval required for paraffin sections.

Immunogen

A synthetic peptide conjugated with KLH has been derived from Rabbit Factor VIII:85-190/2351. To provide a similar yet distinct variation of the given content, the generated text will be based on the original information present without following the format utilized by ChapGPT for conversational dialogue.

Properties

Concentration

1mg/ml

Purification

affinity purified by Protein A

Clonality

Polyclonal Antibody

Isotype

IgG

Stability & Storage

To ensure optimum product preservation, it is recommended that you store at a temperature of -20 °C for a period of one year. It is important to avoid any repeated freeze/thaw cycles to maintain the integrity of the product. Therefore, make sure to handle and store the product in a suitable environment, at the recommended temperature, and follow the storage instructions carefully. This will ensure the product's quality and effectiveness for its intended use.

Storage buffer

A solution of 0.01M Tris-buffered saline at pH7.4 containing 1% bovine serum albumin, 0.03% Proclin300 and 50% glycerol has been prepared.

Research area

Vascular endothelial cells play a crucial role in tumor cell biology as they contribute to cardiovascular signal transduction. These specialized cells express a variety of cell surface molecules that act as markers for different cell types within tumors. By studying these cell type markers, researchers can identify specific subpopulations of tumor cells and gain insights into the molecular mechanisms underlying tumor development and progression. Furthermore, investigating the signaling pathways mediated by these cell surface molecules can provide valuable information on the interactions between tumor cells and their microenvironment. Consequently, by identifying and characterizing tumor cell biomarkers, scientists can develop targeted therapies that specifically intervene in the signaling networks associated with tumor growth and metastasis.

Target

Background

This gene encodes coagulation factor VIII, which participates in the intrinsic pathway of blood coagulation; factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids, converts factor X to the activated form Xa. This gene produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein, isoform a, which circulates in plasma and associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a putative small protein, isoform b, which consists primarily of the phospholipid binding domain of factor VIIIc. This binding domain is essential for coagulant activity. Defects in this gene results in hemophilia A, a common recessive X-linked coagulation disorder. [provided by RefSeq, Jul 2008].

Celluar localization

Extracellular matrix secreted protein

UniPort

P00451