Rabbit Anti-Cytochrome C Polyclonal Antibody #abs130083

abs130083-50ug

1-2 Weeks

201

abs130083-100ug

1-2 Weeks

301

Description

The CYCS protein, an electron carrier, is part of the cytochrome c family. In its oxidized form, the heme group of cytochrome c can receive an electron from the cytochrome c1 subunit of cytochrome reductase. From there, cytochrome c transfers this electron to the cytochrome oxidase complex, which serves as the final protein carrier in the electron-transport chain within the mitochondria.

Other names

Here are some options:
1. The proteins CYC, CYCS, HCS, THC4, and CYC_HUMAN are all related to the molecule cytochrome c. Cytochrome c is involved in cellular respiration and serves as an electron carrier in the electron transport chain.
2. Cytochrome c is a vital molecule in cellular respiration, and several proteins are associated with its function. These include CYC, CYC_HUMAN, CYCS, and HCS. THC4 is also related to cytochrome c, although its exact function is still being studied.
3. There are several proteins that are linked to the function of cytochrome c, including CYC, CYC_HUMAN, CYCS, HCS, and THC4. Cytochrome c is important for cellular respiration, helping to generate energy by transferring electrons along the electron transport chain.
4. CYC, CYC_HUMAN, CYCS, HCS, and THC4 are all proteins associated with cytochrome c, a molecule involved in cellular respiration. Cytochrome c functions as an electron carrier, shuttling electrons along the electron transport chain to generate ATP, the cell's primary energy source.
5. The molecular components CYC, CYC_HUMAN, CYCS, HCS, and THC4 all play a role in the function of cytochrome c, a molecule crucial for cellular respiration. By transferring electrons along the electron transport chain, cytochrome c helps to generate ATP, which powers cellular processes.

Source

Rabbit

Specificity

The detection of endogenous levels of total Cytochrome c can be achieved using the Cytochrome c antibody. This antibody has the ability to specifically target and bind to Cytochrome c, allowing for its detection in biological samples.

Species Reactivity

Human;Mouse;Rat;Monkey

Application

The recommended dilution for using this antibody in Western blotting ranges from 1:500 to 1:3000. For immunohistochemistry, a dilution of 1:50 to 1:200 is recommended. The recommended dilution range for using this antibody in indirect immunofluorescence or immunocytochemistry is 1:100 to 1:500. When using this antibody in ELISA assays, the recommended dilution range for the peptide is 1:20000 to 1:40000. It is important to follow the recommended dilution ranges for optimal results with this antibody.

Immunogen

Human Cytochrome c can be synthesized into a peptide, which is derived using advanced techniques. This synthesized peptide closely resembles the original Cytochrome c and retains its functional properties. The process involves rearranging specific amino acids to create a highly similar and bioactive molecule. This synthesized peptide holds promise for various applications, including biomedical research and therapeutic interventions. It is a testament to the remarkable advancements in peptide synthesis technology and our understanding of the structure-function relationship in human proteins.

Properties

Concentration

1mg/ml

Purification

Using SulfoLink™ Coupling Resin, the antiserum underwent purification through peptide affinity chromatography. To obtain high-quality results, this method was chosen to ensure the efficient removal of impurities and the isolation of the desired antibodies.

Clonality

Polyclonal Antibody

Stability & Storage

The recommended storage condition is to store it at a temperature of -20 °C for one year. It is important to avoid subjecting it to repeated freeze/thaw cycles during storage.

Storage buffer

The Rabbit IgG is preserved in a solution composed of phosphate buffered saline with a pH of 7.4. It contains 150mM NaCl as well as 0.02% sodium azide and 50% glycerol. To maintain its stability, it should be stored at a temperature of -20 °C. This product remains stable for a duration of 12 months from the date of its receipt.

Target

Background

The cytochrome c molecule, an electron carrier protein, plays a crucial role in the mitochondrial electron-transport chain. It can accept an electron from the heme group of the cytochrome c1 subunit found in cytochrome reductase when it is in its oxidized form. Subsequently, this electron is then passed on by cytochrome c to the cytochrome oxidase complex, which acts as the final protein carrier in the electron-transport chain within the mitochondria. By undergoing this sequential electron transfer, the electron carrier protein cytochrome c facilitates the smooth flow of electrons and contributes to the overall functioning of the mitochondria.

Posttranslational modification

Each subunit of this protein binds one heme group. When Tyr-49 or Tyr-98 are phosphorylated, the turnover rate in the reaction with cytochrome c oxidase is reduced by 50%, which has the effect of down-regulating mitochondrial respiration. By modulating the activity of this protein, cells can regulate energy production to meet their needs.

Celluar localization

Cytosol;Mitochondrion;Nucleus;

UniPort

P99999