Rabbit Anti-Desmin Polyclonal Antibody #abs130084

abs130084-50ug

1-2 Weeks

201

abs130084-100ug

1-2 Weeks

301

Description

Desmin is a type of class-III intermediate filaments that can be found within muscle cells. In mature striated muscle, these filaments create a strong fibrous network that connects myofibrils together and links them to the plasma membrane, specifically originating from the periphery of the Z-line structures. However, when mutations occur in this particular gene, it leads to the development of desmin-related myopathy. This inherited condition affects both the cardiac and skeletal muscles, resulting in a disorder known as familial cardiac and skeletal myopathy (CSM). Additionally, these gene mutations have also been associated with distal myopathies, which further emphasizes their significance in muscle-related disorders.

Other names

Desmin, also known as DES or DESM_HUMAN, is an intriguing intermediate filament protein. It is encoded by various genes such as CMD1I, CSM1, CSM2, FLJ12025, FLJ39719, FLJ41013, and FLJ41793. One notable feature of Desmin is its crucial role in maintaining the structural integrity of muscle cells. This unique protein forms a network that connects the different components of the cytoskeleton, providing mechanical support. Its presence is particularly significant in muscles, where it helps in transmitting force and stabilizing the muscle fibers during contraction. The importance of Desmin is evident in its association with certain diseases, including cardiomyopathy and myofibrillar myopathy. Therefore, studying Desmin and unraveling its functions can provide valuable insights into the intricacies of muscle physiology and pathology. It is interesting to note that Desmin is sometimes referred to as FLJ12025, FLJ39719, FLJ41013, FLJ41793, or even OTTHUMP00000064865. This highlights the complexity and diversity of the scientific nomenclature associated with this protein.

Source

Rabbit

Specificity

The Desmin antibody is able to detect the total Desmin protein at its natural levels within the body. To rephrase this, one could say: The total Desmin protein present in the body can be accurately identified using the Desmin antibody.

Species Reactivity

Human;Mouse;Rat

Application

For Western blot analysis, a recommended dilution range would be 1:500 to 1:3000. When it comes to immunohistochemistry, the optimal range would be 1:50 to 1:200. As for ELISA using peptide, it is suggested to dilute the sample at a range of 1:20000 to 1:40000 for best results. It is important to note that these dilution ranges are based on the original text information and should be followed accordingly for accurate results.

Immunogen

A synthesized peptide derived from human Desmin.

Properties

Concentration

1mg/ml

Purification

Using SulfoLink™ Coupling Resin, the antiserum underwent purification through peptide affinity chromatography. A highly purified form of the antiserum was obtained through this process.

Clonality

Polyclonal Antibody

Stability & Storage

To maintain the quality of the product, it is recommended to store it in a freezer at a temperature of -20 °C for a period of one year. It is advised to avoid any repeated instances of thawing and freezing as it can negatively impact the integrity of the product. Taking adequate precautions and following the storage recommendations will help in preserving the product's efficacy for longer periods.

Storage buffer

：IgGpH7.4，150mM、0.02％50％。-20°C，12。

Target

Background

Desmin is a type of class-III intermediate filaments that can be found in muscle cells. Within adult striated muscle, these filaments form a fibrous network that connects myofibrils together and links them to the plasma membrane, starting from the periphery of the Z-line structures. This information can be found in various scientific articles, such as those listed in PubMed under the references PubMed:24200904, PubMed:25394388, and PubMed:26724190.
Interestingly, desmin may also serve as a protein that anchors sarcomeric microtubules. More specifically, it associates with detyrosinated tubulin-alpha chains, leading to the formation of buckled microtubules. This results in increased mechanical resistance to contraction within the muscle cells. This additional function of desmin as a sarcomeric microtubule-anchoring protein is a fascinating aspect that has been observed and studied.

Posttranslational modification

ADP-ribosylation prevents ability to form intermediate filaments.

Celluar localization

Cytoskeleton;Cytosol;Extracellular region or secreted;Plasma Membrane;

UniPort

P17661