Rabbit Anti-DNA Polymerase Beta Polyclonal Antibody #abs130085

abs130085-50ug

1-2 Weeks

201

abs130085-100ug

1-2 Weeks

301

Description

The POLB Repair polymerase is a highly unique DNA polymerase that operates in a different manner than most other DNA polymerases. It facilitates gap-filling DNA synthesis in a stepwise, distributive manner rather than a processive fashion as with other DNA polymerases. Additionally, this polymerase possesses a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity. POLB Repair belongs to the DNA polymerase type-X family and functions as a monomer. Overall, this polymerase plays a vital role in DNA repair processes and helps maintain genomic integrity.

Other names

Beta DNA polymerase, also known as DNA pol beta or DNA polymerase beta, is an essential enzyme involved in DNA replication and repair. It plays a crucial role in maintaining the integrity of the genome. The protein encoded by the DPOLB_HUMAN gene, also referred to as Pol B or Pol beta, is the subunit responsible for the catalytic activity of this polymerase.
DNA polymerase beta is a highly conserved enzyme found in many species, including humans. It is involved in base excision repair, which is a mechanism for fixing damaged DNA. This repair process specifically addresses small-scale DNA lesions, such as those caused by oxidative stress.
The POLB gene, also known as MGC125976, encodes for the DNA polymerase beta protein. It is located on human chromosome 8 and is composed of several exons. The expression of POLB is regulated in a tissue-specific manner.
The absence or malfunction of DNA polymerase beta can lead to various genetic disorders and cancer. Scientists have extensively studied this enzyme to understand its structure and function. It has been a subject of research for developing targeted therapies and drugs that can modulate its activity.
In conclusion, DNA polymerase beta is a vital enzyme involved in DNA replication and repair. Its subunit, encoded by the POLB gene, is responsible for catalyzing the polymerization of DNA molecules. Understanding the mechanisms and regulation of this enzyme is crucial for maintaining genomic stability and preventing diseases related to DNA damage.

Source

Rabbit

Specificity

Total DNA Polymerase beta can be detected by the DNA Polymerase beta antibody, which is able to recognize endogenous levels of the enzyme.

Species Reactivity

Human;Mouse;Rat

Predictive reaction species

Zebrafish;Chicken;Rabbit;Xenopus;Dog;Bovine;Sheep;Pig;

Application

For Western blotting, the recommended dilution ranges from 1:500 to 1:3000. For immunohistochemistry, a dilution range of 1:50 to 1:200 is recommended, while for immunofluorescence and immunocytochemistry, a range of 1:100 to 1:500 is recommended. When performing ELISA with a peptide, a dilution range of 1:20000 to 1:40000 is suggested. It's important to note that these dilution ranges are based on the original text information and should be followed for optimal results.

Immunogen

A man-made peptide has been developed through the use of human DNA Polymerase beta. The synthesized peptide is similar in structure and composition to the original DNA polymerase beta protein.

Properties

Concentration

1mg/ml

Purification

SulfoLink™ Coupling Resin was employed to purify the antiserum through peptide affinity chromatography.

Clonality

Polyclonal Antibody

Stability & Storage

Make sure to store it at -20 °C for a duration of one year, while avoiding any instances of freezing and thawing cycles.

Storage buffer

The storage conditions for Rabbit IgG are as follows: it should be kept in phosphate buffered saline with a pH of 7.4, containing 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is advised to store it at a temperature of -20 °C. The stability of the IgG is guaranteed for a period of 12 months from the date of receipt.

Target

Background

The repair polymerase responsible for base-excision repair is equipped with 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity. This enzymatic activity is crucial as it eliminates the 5' sugar phosphate from the damaged DNA strand. Additionally, this polymerase also functions as a DNA polymerase, allowing it to add a single nucleotide to the 3' end of the resulting single-nucleotide gap. However, unlike other DNA polymerases that perform continuous synthesis, this repair polymerase conducts its gap-filling DNA synthesis in a stepwise distributive manner. It progresses through the repair process by adding nucleotides individually rather than in a continuous and efficient manner.

Posttranslational modification

Methylation by PRMT6 stimulates the polymerase activity by enhancing DNA binding and processivity.Ubiquitinated at Lys-41, Lys-61 and Lys-81: monoubiquitinated by HUWE1/ARF-BP1. Monoubiquitinated protein is then the target of STUB1/CHIP, which catalyzes polyubiquitination from monoubiquitin, leading to degradation by the proteasome. USP47 mediates the deubiquitination of monoubiquitinated protein, preventing polyubiquitination by STUB1/CHIP and its subsequent degradation.

Celluar localization

Cytoskeleton;Nucleus;

UniPort

P06746