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Rabbit Anti-PPAR-gamma Polyclonal Antibody#abs131803

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Description

Catalog-specification	Delivery time	USD price
abs131803-100ug	1-2 Weeks	301.0
abs131803-50ug	1-2 Weeks	201.0

Overview
catalog	abs131803
Description	This specific gene is responsible for producing a particular type of protein that belongs to the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs work by forming heterodimers with retinoid X receptors (RXRs), a process that plays a significant role in regulating gene transcription. Typically, there are three subtypes of PPARs, which include PPAR-alpha, PPAR-delta, and PPAR-gamma.
Other names	Nuclear receptor subfamily 1 group C member 3, also known as Peroxisome Proliferator Activated Receptor gamma (PPAR gamma) or PPARG, is a protein that in humans is encoded by the PPARG gene. There are two known isoforms of PPAR gamma: PPARG1 and PPARG2. PPAR gamma is a transcription factor that plays a crucial role in adipocyte differentiation and glucose homeostasis. It is also involved in the regulation of inflammation and cellular growth. PPAR gamma is the target of thiazolidinedione drugs used to treat type 2 diabetes. Variations in the PPARG gene are associated with an increased risk of susceptibility to metabolic diseases. Furthermore, PPAR gamma is highly expressed in adipose tissue and has been implicated in the pathophysiology of obesity. In conclusion, PPAR gamma is a vital protein that regulates gene expression and metabolic homeostasis, making it an attractive target for therapeutic intervention.
Source	Rabbit
Specificity	Detecting endogenous levels of total PPAR-gamma, the PPAR-gamma Antibody offers a reliable method. By rearranging the original content, it emphasizes the ability of the antibody to accurately measure the overall presence of PPAR-gamma in a sample.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Rabbit;Dog;Horse
Antigen	PPAR-gamma
Application	For western blotting, the recommended dilution range is 1:500-1:2000. The dilution range for immunohistochemistry is 1:50-1:200. For immunofluorescence/immunocytochemistry, the recommended dilution range is 1:100-1:500. When using ELISA with peptide, the suggested dilution range is 1:20000-1:40000. It is essential to use the recommended dilution range to ensure optimal results.
Immunogen	One potential form of therapeutic peptide is synthesized from human PPAR-gamma. This peptide has the ability to bind to and activate the PPAR-gamma receptor, which plays a crucial role in regulating glucose and lipid metabolism. By targeting this receptor, the synthesized peptide may offer potential benefits for individuals with metabolic diseases such as insulin resistance and type 2 diabetes. Further research is needed to fully explore the therapeutic potential of this peptide and optimize its development for clinical use.
MW	57kDa
Properties
Concentration	1mg/ml
purification	Utilizing SulfoLink™ Coupling Resin, the antiserum underwent purification via peptide affinity chromatography. For this process, the resin was coupled with peptides that have an affinity for the antiserum, allowing for impurities to be removed and the final product to be highly purified.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. ARF6 acts as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated proinflammatory responses. Plays a role in the regulation of cardiovascular circadian rhythms by regulating the transcription of ARNTL/BMAL1 in the blood vessels (By similarity).
Tissue specificity	Highest expression in adipose tissue. Lower in skeletal muscle, spleen, heart and liver. Also detectable in placenta, lung and ovary.
Posttranslational modification	O-GlcNAcylation at Thr-84 reduces transcriptional activity in adipocytes.Phosphorylated in basal conditions and dephosphorylated when treated with the ligand. May be dephosphorylated by PPP5C. The phosphorylated form may be inactive and dephosphorylation at Ser-112 induces adipogenic activity (By similarity).
Celluar localization	Cytosol;Nucleus;
UniPort	P37231