Rabbit Anti-SHIP1 Polyclonal Antibody #abs130188

abs130188-50ug

1-2 Weeks

201

abs130188-100ug

1-2 Weeks

301

Description

SHIP is a phosphatase that contains SH2 and regulates various cellular processes, including cell survival, growth, cell cycle arrest, and apoptosis. This hemopoietic-specific protein hydrolyzes Ins(1,3,4,5)P4 and PtdIns(3,4,5)P3. It is localized in the cytosol and possesses an SH2 domain at its N-terminus and two NPXY Shc binding motifs at its C-terminus.

Other names

The inositol polyphosphate 5 phosphatase of 145kDa, also known as SHIP-1, is an important enzyme involved in regulating cellular signaling pathways. It is encoded by the INPP5D gene and plays a key role in phosphatidylinositol signaling. The enzyme is also referred to as 4,5-trisphosphate 5-phosphatase 1 or Phosphatidylinositol-3 and has a molecular weight of 145kDa. Other aliases include hp51CN, p150Ship, and SIP-145. The protein contains an SH2 domain, which enables it to interact with other signaling proteins. Mutations in the INPP5D gene have been associated with various diseases, including cancer and autoimmune disorders. The precise function of SHIP-1 is still being investigated, but it is known to be involved in modulating immune cell activation, insulin signaling, and platelet activation.

Source

Rabbit

Specificity

The SHIP1 antibody effectively recognizes and quantifies the total amount of naturally occurring SHIP1 protein in biological samples. This indicates that the antibody has the ability to bind specifically to endogenous SHIP1 and can be used in various experiments to study its expression level and function.

Species Reactivity

Human;Mouse;Rat

Predictive reaction species

Pig;Rabbit;Sheep;Dog;Horse;Bovine;

Application

：
WB: 1:500~1:3000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000.
：
IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000, WB: 1:500~1:3000.
：
ELISA (peptide)1:20000-1:40000，IF/ICC1:100-1:500，WB1:500~1:3000。

Immunogen

A synthesized peptide derived from human SHIP1.

Properties

Concentration

1mg/ml

Purification

Through the application of SulfoLink™ Coupling Resin, a peptide affinity chromatography technique was employed to purify the antiserum.

Clonality

Polyclonal Antibody

Stability & Storage

To ensure the preservation of the contents, it is recommended to store them at -20 °C for a period of one year. It is important to avoid subjecting them to repeated freeze/thaw cycles.

Storage buffer

This product contains Rabbit IgG in a buffered saline solution with a pH of 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be stored at -20°C and is stable for up to one year from the date of receipt.

Target

Background

Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways. Acts as a negative regulator of B-cell antigen receptor signaling. Mediates signaling from the FC-gamma-RIIB receptor (FCGR2B), playing a central role in terminating signal transduction from activating immune/hematopoietic cell receptor systems. Acts as a negative regulator of myeloid cell proliferation/survival and chemotaxis, mast cell degranulation, immune cells homeostasis, integrin alpha-IIb/beta-3 signaling in platelets and JNK signaling in B-cells. Regulates proliferation of osteoclast precursors, macrophage programming, phagocytosis and activation and is required for endotoxin tolerance. Involved in the control of cell-cell junctions, CD32a signaling in neutrophils and modulation of EGF-induced phospholipase C activity. Key regulator of neutrophil migration, by governing the formation of the leading edge and polarization required for chemotaxis. Modulates FCGR3/CD16-mediated cytotoxicity in NK cells. Mediates the activin/TGF-beta-induced apoptosis through its Smad-dependent expression. May also hydrolyze PtdIns(1,3,4,5)P4, and could thus affect the levels of the higher inositol polyphosphates like InsP6.

Tissue specificity

Specifically expressed in immune and hematopoietic cells. Expressed in bone marrow and blood cells. Levels vary considerably within this compartment. Present in at least 74% of immature CD34+ cells, whereas within the more mature population of CD33+ cells, it is present in only 10% of cells. Present in the majority of T-cells, while it is present in a minority of B-cells (at protein level).

Posttranslational modification

Tyrosine phosphorylated by the members of the SRC family after exposure to a diverse array of extracellular stimuli such as cytokines, growth factors, antibodies, chemokines, integrin ligands and hypertonic and oxidative stress. Phosphorylated upon IgG receptor FCGR2B-binding.

Celluar localization

Cytoskeleton;Cytosol;Plasma Membrane;

UniPort

Q92835