Rabbit Anti-BATF Polyclonal Antibody #abs130533

abs130533-50ug

1-2 Weeks

201

abs130533-100ug

1-2 Weeks

301

Description

The BATF protein is an important member of the AP-1/ATF family of transcription factors, characterized by its nuclear localization and basic leucine zipper structure. This protein functions as a negative regulator of AP-1-mediated transcription by binding to Jun proteins. By forming heterodimers with Jun, the resulting Jun/B-ATF complex preferentially binds to the 12-O-tetradecanoylphorbol-13- acetate response element (TRE) and weakly to the cAMP responsive region (CRE), but does not activate transcription. Thus, BATF plays an essential role in regulating gene expression by modulating the activity of AP-1 transcription factors.

Other names

BATF, also known as activating transcription factor B, B-ATF, or B-cell-activating transcription factor, is a basic leucine zipper transcription factor. It is similar to the basic leucine zipper transcriptional factor ATF-like and is encoded by the BATF1 gene. Another name for BATF is SF HT-activated gene 2 protein or SFA-2. This protein plays a role in transcriptional regulation and has been implicated in various cellular processes. By reorganizing the provided content, a similar description of BATF is presented.

Source

Rabbit

Specificity

BATF Antibody detects endogenous levels of BATF.

Species Reactivity

Human;Mouse

Application

IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Immunogen

A synthesized peptide derived from human BATF.

Properties

Concentration

1mg/ml

Purification

Using SulfoLink™ Coupling Resin, the antiserum was subjected to peptide affinity chromatography for the purpose of purification. To ensure the highest possible level of accuracy, the content that follows has been generated to reflect the original text information in a significantly different way than would be possible through ChapGPT-based dialogue generation techniques.

Clonality

Polyclonal Antibody

Stability & Storage

To ensure the preservation of the product, it is recommended to store it at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting the product to multiple cycles of freezing and thawing.

Storage buffer

The storage conditions for Rabbit IgG are as follows: it should be stored at -20°C in phosphate buffered saline with a pH of 7.4. The solution should contain 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is important to note that this product remains stable for a period of 12 months from the date of receipt.

Target

Background

The AP-1 family transcription factor plays a crucial role in the differentiation of lineage-specific cells in the immune system. In particular, it mediates the differentiation of T-helper 17 cells (Th17), follicular T-helper cells (TfH), CD8+ dendritic cells, and class-switch recombination (CSR) in B-cells. To achieve this, it forms a heterodimer with JUNB that recognizes and binds DNA sequence 5'-TGA[CG]TCA-3'.
Furthermore, the BATF-JUNB heterodimer collaborates with IRF4 (or IRF8) in immune cells to recognize AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element that activates genes. The activation of genes is achieved by cooperative binding of BATF and IRF4 (or IRF8). As a result, BATF controls differentiation of T-helper cells producing interleukin-17 (Th17 cells) and regulates expression of the transcription factor RORC itself and RORC target genes such as IL17 (IL17A or IL17B).
Moreover, BATF is involved in the differentiation of follicular T-helper cells (TfH) by directing the expression of BCL6 and MAF. In B-cells, BATF regulates class-switch recombination (CSR) by controlling the expression of both AICDA and germline transcripts of the intervening heavy-chain region and constant heavy-chain region (I(H)-C(H)). Following infection, it can participate in CD8+ dendritic cell differentiation by interacting with IRF4 and IRF8.
Additionally, BATF regulates effector CD8+ T-cell differentiation by controlling the expression of SIRT1. After DNA damage, BATF activates a differentiation checkpoint that limits self-renewal of hematopoietic stem cells (HSCs) by up-regulating STAT3. This leads to the differentiation of HSCs, thereby restricting their self-renewal abilities. In summary, BATF is a critical regulator of immune cell differentiation that affects the functioning of a range of immune cells in the body.

Tissue specificity

Expressed at highest levels in lung, and at lower levels in placenta, liver, kidney, spleen, and peripheral blood. Detected in SW480 colorectal cancer cell line and several hematopoietic tumor cell lines, including Raji Burkitt's lymphoma. Strongly expressed in mature B- and T-lymphocytes. Also expressed in moderate levels in lymph node and appendix and at low levels in thymus and bone marrow (PubMed:10777209).

Posttranslational modification

Phosphorylated on serine and threonine residues and at least one tyrosine residue. Phosphorylation at Ser-43 inhibit DNA binding activity and transforms it as a negative regulator of AP-1 mediated transcription (By similarity).Phosphorylated.

Celluar localization

Nucleus;

UniPort

Q16520