Rabbit Anti-Uba2 Polyclonal Antibody #abs130223

abs130223-50ug

1-2 Weeks

201

abs130223-100ug

1-2 Weeks

301

Description

SAE2 belongs to the family of ubiquitin-activating E1 proteins and serves as a ligase for UBL1. It plays a significant role in the activation of UBL1 by utilizing ATP-dependent mechanisms and facilitates the formation of a thiolester bond with a cysteine residue that is conserved on SAE2.

Other names

ARX, also known as anthracycline-associated resistance, is a protein encoded by the UBA2 gene in humans. It is also referred to as FLJ13058, HRIHFB2115, SAE2, SAE2_HUMAN, SUMO 1 activating enzyme subunit 2, SUMO activating enzyme subunit 2, and SUMO-activating enzyme subunit 2.
ARX plays a critical role in the cellular response to anthracycline-based chemotherapy, which is commonly used to treat a variety of cancers. It is involved in the activation of the small ubiquitin-like modifier (SUMO) family of proteins, which regulate a wide range of cellular processes. These proteins are often dysregulated in cancer, leading to altered cell signaling and resistance to chemotherapy.
ARX has also been implicated in other biological processes, including DNA damage repair and innate immunity. It interacts with several other proteins involved in these pathways, suggesting that it may have broader functions beyond its role in chemotherapy resistance.
Overall, ARX is an important protein that regulates a variety of cellular processes and has implications for cancer treatment and other disease states. Further research is needed to fully characterize its roles and potential therapeutic applications.

Source

Rabbit

Specificity

The detection of endogenous levels of total Uba2 can be achieved using the Uba2 antibody. This antibody specifically targets and recognizes Uba2, allowing for the measurement of its expression in biological samples.

Species Reactivity

Human

Application

For Western Blot, the recommended dilution range for our antibody is 1:500 to 1:3000. When used for Immunohistochemistry, we suggest diluting our antibody to 1:50 to 1:200. For Immunofluorescence and Immunocytochemistry, a dilution of 1:100 to 1:500 is recommended. When using our peptide for ELISA, we suggest a dilution range of 1:20000 to 1:40000. These dilution ranges are based on our testing and have been proven effective for our antibody and peptide products.

Immunogen

A synthesized peptide derived from human Uba2.

Properties

Concentration

1mg/ml

Purification

SulfoLink™ Coupling Resin was utilized for peptide affinity chromatography to purify the antiserum.

Clonality

Polyclonal Antibody

Stability & Storage

To ensure longevity, it is recommended to store at a temperature of -20 °C for a duration of one year. It is important to avoid exposing it to repetitive cycles of freezing and thawing. Take necessary precautions to maintain its quality and functionality.

Storage buffer

To ensure optimal storage and stability, this Rabbit IgG should be stored at -20°C in phosphate buffered saline with a pH of 7.4 and a concentration of 150mM NaCl. The solution also contains 0.02% sodium azide and 50% glycerol to prevent degradation and microbial growth. This product has a shelf life of 12 months from the date of receipt, so it is important to monitor expiration dates and replace with fresh stock accordingly. By following proper storage guidelines, you can maintain the integrity and activity of the Rabbit IgG.

Target

Background

The UBA2/SAE2 heterodimer is responsible for catalyzing the conjugation of SUMO proteins, including SUMO1, SUMO2, SUMO3, and possibly SUMO4. This process requires ATP-dependent activation of the SUMO protein and the formation of a thioester bond between the SUMO protein and a specific cysteine residue located within the active site of UBA2/SAE2. Through this activity, the UBA2/SAE2 heterodimer serves as an E1 ligase for the SUMO family of proteins.

Posttranslational modification

Sumoylated with SUMO1 and SUMO2/3 and by UBC9. Sumoylation at Lys-236 inhibits enzymatic activity. Sumoylation at the C-terminal lysine cluster plays an essential role in nuclear trafficking.

Celluar localization

Nucleus;

UniPort

Q9UBT2