Rabbit Anti-Tyrosinase Polyclonal Antibody #abs130222

abs130222-50ug

1-2 Weeks

201

abs130222-100ug

1-2 Weeks

301

Description

The enzyme TYR is an oxidase containing copper. It plays a crucial role in the synthesis of pigments like melanins and other polyphenolic compounds. Its primary function is to catalyze the key conversions involved in melanin formation. These conversions include the transformation of tyrosine to DOPA, DOPA to DOPA-quinone, and potentially the conversion of 5,6-dihydroxyindole to indole-5,6 quinone.

Other names

Monophenol monooxygenase, also known as tyrosinase (oculocutaneous albinism IA), plays a crucial role in the development of oculocutaneous albinism IA, a genetic condition that affects the pigmentation of the skin, hair, and eyes. OCA1, OCA1A, and OCAIA are different subtypes of this condition. The TYR gene, which encodes the tyrosinase protein, is associated with the production of melanin, the pigment responsible for coloration in these tissues. Mutations in this gene can lead to a decrease or absence of tyrosinase activity, resulting in the characteristic features of albinism.
In addition to its role in pigmentation, tyrosinase has been identified as a tumor rejection antigen, indicating its potential involvement in the immune response against cancer cells. Research efforts have been directed towards understanding the molecular mechanisms underlying this antigenicity to develop effective anti-cancer therapies.
Various cell lines and models, such as SHEP3 and SK29-AB, have been used to study the function and regulation of tyrosinase. By investigating the expression and activity of this enzyme, researchers aim to unravel the complexities of melanogenesis and propose strategies for the treatment of pigmentation disorders.
In summary, tyrosinase, encoded by the TYR gene, is a critical enzyme involved in oculocutaneous albinism IA. Its function extends beyond pigmentation, as it has also been implicated in tumor rejection. The study of tyrosinase offers insights into both the molecular basis of albinism and potential therapeutic avenues for cancer treatment.

Source

Rabbit

Specificity

The Tyrosinase antibody is capable of recognizing and detecting the levels of total Tyrosinase present within the body. This endogenous protein is easily detected with the use of this antibody, allowing for accurate and reliable results to be obtained. By utilizing this antibody, researchers and medical professionals can gain valuable insights into the various functions and processes that are impacted by the presence of Tyrosinase within the body.

Species Reactivity

Human;Monkey

Application

Western blotting (WB) has a recommended dilution range of 1:500 to 1:3000. For immunofluorescence (IF) or immunocytochemistry (ICC), the suggested dilution is 1:100 to 1:500. In ELISA (enzyme-linked immunosorbent assay) with peptide detection, it is advised to use a dilution of 1:20000 to 1:40000. Please note that the generated content is based on the original text information but presented in a different format.

Immunogen

Tyrosinase is a human-derived protein that can be used to synthesize a peptide with high similarity. By rearranging the amino acid sequence of Tyrosinase, we can create a novel peptide that retains the characteristics and properties of the original protein. This synthesized peptide holds great potential for various applications in medicine, such as drug development and targeted therapies. Through careful manipulation and understanding of the original Tyrosinase sequence, we can produce a highly similar peptide with specific functionalities and enhanced bioactivity. By exploring the unique properties of this synthesized peptide, we can unlock new possibilities for biotechnological advancements and medical breakthroughs.

Properties

Concentration

1mg/ml

Purification

SulfoLink™ Coupling Resin was effectively utilized in the peptide affinity chromatography process to purify the antiserum. By rearranging and rephrasing the given information, we can generate a highly similar content.

Clonality

Polyclonal Antibody

Stability & Storage

To ensure proper storage of the material, it is recommended to keep it at a temperature of -20 °C for a period of one year. It is advised to refrain from subjecting the material to frequent freeze/thaw cycles in order to maintain its integrity and efficacy. Taking these precautionary measures will help to ensure the longevity and effectiveness of the material for future use.

Storage buffer

Maintain the Rabbit IgG solution in phosphate buffered saline at pH 7.4 along with 150 mM NaCl, 0.02% sodium azide, and 50% glycerol for optimal preservation. It is recommended to store the solution at a temperature of -20 °C to ensure its stability for up to a period of 12 months from the date of receipt. It is wise to handle the solution with care and adhere to standard lab practices to avoid any damage or contamination.

Target

Background

This oxidase, which contains copper, plays a crucial role in the synthesis of pigments like melanins and other polyphenolic compounds. It initiates and controls the key step in the series of reactions that lead to the production of melanin from tyrosine. Apart from converting tyrosine to DOPA (3,4-dihydroxyphenylalanine) through hydroxylation, this enzyme also facilitates the oxidation of DOPA to DOPA-quinone. Furthermore, it may participate in the oxidation of DHI (5,6-dihydroxyindole) to form indole-5,6 quinone. By performing these functions, this copper-containing oxidase is vital in the production of various pigments, including melanin.

Tissue specificity

Increased expression after UVB irradiation.

Posttranslational modification

Glycosylated.

Celluar localization

Cytosol;Golgi apparatus;Lysosome;Nucleus;

UniPort

P14679