Rabbit Anti-mGluR6 Polyclonal Antibody #abs130107

abs130107-50ug

1-2 Weeks

201

abs130107-100ug

1-2 Weeks

301

Description

The glutamate receptor mGluR6 is responsible for receiving and transmitting signals related to glutamate. Its activity is regulated by a G-protein, which plays a role in inhibiting the activity of adenylate cyclase. This receptor belongs to the G-protein coupled receptor 3 family. To emphasize, please note that the speech generated will be markedly different from the original text as per your request.

Other names

Metabotropic glutamate receptor 6, also known as Glutamate receptor metabotropic 6 or mGluR6, is a protein encoded by the GRM6 gene in humans. It is a member of the class C G protein-coupled receptor family and is primarily expressed in the retina of the eye. Its main function is to regulate synaptic transmission between photoreceptor cells and bipolar cells in the retina, which is crucial for visual processing. Other names for this protein include Grm6, GPRC1F, GluR6, and CSNB1B. DKFZp686H1993 is the name of the gene's corresponding cDNA clone.

Source

Rabbit

Specificity

The mGluR6 antibody is designed to recognize and target all forms of endogenous mGluR6. By binding to this protein, the antibody can serve as a valuable tool for studying its various functions and interactions within the cellular environment. Whether you are conducting basic research into the mechanisms of neuronal signaling or exploring new therapeutic avenues for treating neurological disorders, the mGluR6 antibody can help you achieve your goals. With its high specificity and sensitivity, this antibody is a reliable and effective tool for investigating the intricacies of mGluR6 biology.

Species Reactivity

Human

Application

Western Blot (WB) detects proteins in a specific sample at dilutions ranging from 1:500 to 1:3000. Immunohistochemistry (IHC) is performed at dilutions of 1:50 to 1:200 to visualize the presence and distribution of proteins. For ELISA using peptides, a dilution range of 1:20000 to 1:40000 is applied to accurately quantify protein levels. These dilutions are essential for reliable detection and measurement in these experimental techniques.

Immunogen

A synthesized peptide derived from human mGluR6

Properties

Concentration

1mg/ml

Purification

Affinity-chromatography

Clonality

Polyclonal Antibody

Stability & Storage

To maintain the quality of the product, it is recommended to store it at a temperature of -20 °C for a period of one year. It is important to prevent the product from undergoing multiple freeze/thaw cycles as this can affect its efficacy.

Storage buffer

The rabbit IgG is stored in phosphate buffered saline with a pH of 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be kept at a temperature of -20 °C. This product remains stable for a period of 12 months starting from the date of receipt.

Target

Background

Glutamate, a neurotransmitter, binds to a G-protein coupled receptor. This binding event induces a change in the receptor's conformation, leading to downstream signaling through G proteins. Consequently, the activity of adenylate cyclase, an enzyme, is modulated. Specifically, the signaling inhibits adenylate cyclase activity. Additionally, the signaling activates TRPM1 channels and facilitates the uptake of calcium ions. This receptor is essential for maintaining normal vision.

Tissue specificity

Detected in melanocytes.

Celluar localization

The endoplasmic reticulum, Golgi apparatus, and plasma membrane are all essential components of cells. The endoplasmic reticulum is responsible for protein synthesis and lipid metabolism, while the Golgi apparatus plays a crucial role in processing, packaging, and transporting proteins. The plasma membrane, on the other hand, is the outermost barrier of the cell, controlling the flow of materials in and out of the cell.
These three structures work closely together to maintain the proper functioning of the cell. Proteins synthesized on the endoplasmic reticulum are transported to the Golgi apparatus for processing and modification before being sent to their final destination, either within or outside the cell. The plasma membrane acts as the gatekeeper, determining which substances are allowed to pass through into the cell and which are not.
Overall, the endoplasmic reticulum, Golgi apparatus, and plasma membrane are integral to the functioning of cells and must work together in a coordinated fashion to maintain the health and viability of the organism.

UniPort

O15303