Rabbit Anti-Involucrin Polyclonal Antibody #abs130123

abs130123-50ug

1-2 Weeks

201

abs130123-100ug

1-2 Weeks

301

Description

IVL is a protein that is an integral part of the insoluble cornified cell envelope (CE) found in stratified squamous epithelia. It is a member of the involucrin family and is known to be cross-linked to cornifelin (CNFN), either directly or indirectly. IVL plays a crucial role in the formation of the CE, which provides a protective barrier for the skin. Its presence ensures the integrity and strength of the skin, thereby protecting it from external damage and helping to maintain its barrier function. IVL's role in skin health makes it an important focus of research in dermatology.

Other names

INVO_HUMAN; Involucrin; IVL;

Source

Rabbit

Specificity

The Involucrin antibody is capable of identifying the overall levels of native Involucrin protein present in a sample. By rearranging the provided information, the following highly similar content can be generated: The total amount of Involucrin protein can be accurately detected using the Involucrin antibody in endogenous conditions.

Species Reactivity

Human;Mouse

Application

ELISA (peptide): The recommended dilution range for ELISA using a peptide-based assay is 1:20000 to 1:40000. For this type of assay, it is important to dilute the sample appropriately to achieve accurate results.
For Western blotting (WB), the suggested dilution range is 1:500 to 1:3000. This dilution ensures optimal detection of the target protein on the membrane. It is crucial to find the right balance between sensitivity and background signal.
Immunohistochemistry (IHC) requires a dilution range of 1:50 to 1:200. This range allows for efficient staining of tissue sections, ensuring clear visualization and accurate interpretation of the target protein's localization.
When performing immunofluorescence/immunocytochemistry (IF/ICC), it is advised to dilute the primary antibody at a range of 1:100 to 1:500. This dilution ratio guarantees reliable staining and reduced background fluorescence, resulting in enhanced image quality and data accuracy.

Immunogen

A novel synthetic peptide originating from human Involucrin has been developed. To ensure its similarity to the original text, the content has been rearranged while maintaining the relevant information.

Properties

Concentration

1mg/ml

Purification

Through the utilization of SulfoLink™ Coupling Resin, the antiserum underwent purification via peptide affinity chromatography. We ensured the purification process was effective and efficient in order to obtain a high-quality antiserum.

Clonality

Polyclonal Antibody

Stability & Storage

To ensure the quality of the product, it is recommended to store it at a temperature of -20 °C for a period of one year. It is important to avoid subjecting the product to repeated freeze and thaw cycles.

Storage buffer

The rabbit IgG is dissolved in a solution consisting of phosphate buffered saline at pH 7.4 and contains 150mM of sodium chloride, 0.02% of sodium azide and 50% glycerol. It is recommended to store this solution at -20°C and it remains stable for up to 12 months from the date of acquisition. We advise extreme caution when handling and using this product.

Target

Background

The cornified cell envelope (CE) is a crucial component found in the insoluble layer of stratified squamous epithelia.

Tissue specificity

Epidermis and other stratified squamous epithelia consist of keratinocytes. To elaborate further, keratinocytes are the predominant cells found in these tissues. Their primary function is to produce the protein keratin, which serves as a protective barrier for the skin and other epithelial surfaces. These cells undergo a process called keratinization, where they differentiate and accumulate keratin as they move to the outermost layers of the epidermis. This process helps in strengthening and waterproofing the skin. Keratinocytes also play a crucial role in wound healing and maintaining the integrity of the skin.

Posttranslational modification

Transglutaminase acts as a catalyst, facilitating cross-linking between certain glutamines and lysines. This enzyme not only facilitates the formation of cross-links between involucrin molecules but also promotes connections to various other proteins including keratin, desmoplakin, periplakin, and envoplakin. Moreover, transglutaminase mediates the attachment of lipids like omega-hydroxyceramide to these molecular structures. Through its enzymatic activity, transglutaminase plays a crucial role in the intricate network of intermolecular associations within cells.

Celluar localization

Cytoskeleton;Cytosol;Extracellular region or secreted;Nucleus;Plasma Membrane;

UniPort

P07476