Rabbit Anti-TIMP3 Polyclonal Antibody #abs130201

abs130201-50ug

1-2 Weeks

201

abs130201-100ug

1-2 Weeks

301

Description

Tissue inhibitor of metalloproteinases 3 (TIMP3) is involved in the formation of complexes with metalloproteinases, particularly collagenases. These complexes bind to the catalytic zinc cofactor present in the metalloproteinases, leading to their irreversible inactivation. This crucial process is believed to be part of a specific response to remodeling stimuli in various tissues. Notably, TIMP3 has been found to act on several metalloproteinases including MMP1, MMP2, MMP3, MMP7, MMP9, MMP13, MMP14, and MMP15. This interaction and inactivation of metalloproteinases play a significant role in regulating tissue remodeling processes.

Other names

Here are some other variations of the original content:
- Metalloproteinase inhibitor 3, also known as TIMP-3 or tissue inhibitor of metalloproteinases 3, is a protein that plays a key role in controlling the activity of matrix metalloproteinases (MMPs). These enzymes are responsible for breaking down extracellular matrix (ECM) components, such as collagen and elastin, which are important for tissue remodeling and repair. Without proper regulation, MMPs can contribute to tissue damage and disease, including cancer and inflammation. TIMP-3 inhibits MMPs by binding to them and preventing their activity. It is expressed in various tissues, including the eye, where mutations in the TIMP3 gene have been linked to Sorsby fundus dystrophy, a rare genetic disorder that leads to progressive vision loss.
- MIG-5 protein, also known as metalloproteinase inhibitor 3 (TIMP-3), is a member of the tissue inhibitor of metalloproteinases (TIMP) family. TIMPs are endogenous inhibitors of matrix metalloproteinases (MMPs), which are enzymes that degrade extracellular matrix (ECM) proteins. MMPs play a critical role in processes such as tissue remodeling, wound healing, and angiogenesis. However, dysregulated MMP activity can contribute to disease pathology, such as cancer invasion and metastasis. TIMP-3 has a unique role among TIMPs in that it can inhibit the activity of not only MMPs but also other proteases, such as adamalysins and ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs). Its expression is regulated by cytokines and growth factors, and it is involved in diverse physiological processes, including angiogenesis, cell migration, and apoptosis.
- The protein SFD, or Sorsby fundus dystrophy pseudoinflammatory, is a form of tissue inhibitor of metalloproteinases 3 (TIMP-3) that is mutated in cases of Sorsby fundus dystrophy (SFD). SFD is a rare hereditary disease that affects the retina and leads to progressive blindness. The mutated form of TIMP-3 is thought to accumulate in the ECM of the retina and interfere with normal tissue remodeling processes, resulting in the death of photoreceptor cells. The exact mechanisms by which this occurs are not fully understood, but it has been suggested that the mutated TIMP-3 may have altered binding properties or be resistant to degradation. Researchers hope that understanding the biology of SFD will lead to new treatments for retinal diseases.

Source

Rabbit

Specificity

The TIMP3 antibody has the ability to recognize the natural expression of total TIMP3 in a sample. By utilizing this antibody, researchers can effectively identify and study the properties of endogenous TIMP3 levels.

Species Reactivity

Human;Mouse;Rat

Application

For Western blotting, a recommended dilution range is between 1:500 to 1:3000, while for indirect immunofluorescence or immunocytochemistry, the appropriate dilution is between 1:100 to 1:500. In an ELISA assay using peptides, the optimal dilution range is between 1:20000 to 1:40000. These dilutions ensure that the antibodies are used at the appropriate concentrations to provide accurate and reliable results. Therefore, it is important to carefully consider the dilution range for each application to obtain the best possible outcomes.

Immunogen

A synthesized peptide derived from human TIMP3.

Properties

Concentration

1mg/ml

Purification

SulfoLink™ Coupling Resin was utilized for peptide affinity chromatography to purify the antiserum. This process ensured that a high level of purity was achieved in the resulting antiserum.

Clonality

Polyclonal Antibody

Stability & Storage

To ensure preservation, it is advised to store the product at a temperature of -20 °C for a duration of one year. It is crucial to prevent alternating cycles of freezing and thawing.

Storage buffer

This Rabbit IgG product comes in a solution of phosphate buffered saline with a pH of 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended that the product is stored at a temperature of -20°C for up to 12 months from the date of receipt. This product is stable and suitable for a variety of research applications.

Target

Background

Metalloproteinases, including collagenases, can be effectively complexed and irreversibly rendered inactive by binding to their catalytic zinc cofactor. This mechanism of action is thought to be involved in the tissue-specific acute response to remodeling stimuli. Notably, it has been observed that the binding ability of this complex extends to various metalloproteinases such as MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14, and MMP-15.

Celluar localization

Extracellular region or secreted;Nucleus;

UniPort

P35625