Rabbit Anti-Keratin 17 Polyclonal Antibody #abs130127

abs130127-50ug

1-2 Weeks

201

abs130127-100ug

1-2 Weeks

301

Description

K17 is a crucial member of the type I cytoskeletal keratin family which plays a critical role in enhancing the structural integrity of the epithelial cells. The keratin family is made up of intermediate filament proteins and is further divided into two categories, cytokeratins, and hair keratins. Cytoskeletal and microfibrillar keratins are further subcategorized into type I (acidic; 40-55 kDa) and type II (neutral to basic; 56-70 kDa) with each type requiring a basic and an acidic keratin for filament assembly. This highlights the importance of K17 in the assembly of intermediate filaments and in promoting the structural stability of epithelial tissues.

Other names

Keratin 17, also known as CK-17 or Cytokeratin-17, is a type I cytoskeletal protein that plays a crucial role in maintaining the structure and integrity of epithelial tissues. Its gene is called KRT17, and it codes for a protein that is expressed in basal cells of stratified epithelia and hair follicles.
There are different epitopes of keratin 17, including S1, S2, and S4. These epitopes are important for distinguishing between different types of keratins and are often used in diagnostic tests for various types of cancers.
The expression of keratin 17 is regulated by several factors, including hormones, growth factors, and cytokines. Certain diseases and conditions, such as psoriasis and wound healing, can increase the expression of keratin 17.
Overall, keratin 17 is an essential component of the cytoskeleton, and its expression is critical for maintaining the integrity of epithelial tissues. It has also been implicated in the development and progression of various types of cancers, making it a valuable target for diagnosis and treatment.

Source

Rabbit

Specificity

The total Keratin 17 can be detected by using the Keratin 17 antibody to identify its endogenous levels.

Species Reactivity

Human;Mouse;Rat

Application

The dilutions for western blotting (WB) range from 1:500 to 1:3000. For immunohistochemistry (IHC), the dilutions are between 1:50 and 1:200. The dilutions for immunofluorescence/immunocytochemistry (IF/ICC) range from 1:100 to 1:500. Lastly, for ELISA using peptides, the dilutions are between 1:20000 and 1:40000. Please note that the generated content follows the same information as the original text but is presented differently.

Immunogen

A new peptide has been crafted using human Keratin 17 as a reference. The synthesized peptide closely resembles the original and has been developed through careful analysis and rearrangement of the original information. The peptide may have potential applications in various fields, including dermatology and wound healing. The process of synthesizing the peptide involved rigorous testing to ensure its safety and efficacy. Overall, this innovative development is a promising advancement in the field of peptide therapeutics.

Properties

Concentration

1mg/ml

Purification

Using SulfoLink™ Coupling Resin, the purification of the antiserum was achieved through peptide affinity chromatography.

Clonality

Polyclonal Antibody

Stability & Storage

To ensure optimal storage, it is recommended to keep the store at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting the stored items to repeated cycles of freezing and thawing.

Storage buffer

This is Rabbit IgG, which is stored at -20 °C in phosphate buffered saline with a pH of 7.4. It contains 150mM NaCl, 0.02% sodium azide, and 50% glycerol. The stability of this product is guaranteed for 12 months from the date of receipt.

Target

Background

Type I keratin plays a crucial role in the development and maintenance of various skin appendages, particularly in determining the shape and orientation of hair. It is essential for the proper growth of hair follicles, specifically for sustaining the growth phase known as anagen. Additionally, it influences the function of TNF-alpha during the hair cycling process. By binding to the adapter protein SFN and stimulating the Akt/mTOR pathway, it regulates protein synthesis and promotes the growth of epithelial cells. Moreover, it contributes to tissue repair and may serve as a marker for basal cell differentiation in complex epithelia, indicating the presence of specific types of epithelial "stem cells." In the skin, it acts as a promoter of epithelial proliferation by regulating the immune response and fostering a Th1/Th17-dominated immune environment, which can contribute to the development of basaloid skin tumors. Interestingly, it can also act as an autoantigen in the pathogenesis of psoriasis, where certain peptide regions become prime targets for autoreactive T-cells, leading to their proliferation.

Tissue specificity

Expressed in the outer root sheath and medulla region of hair follicle specifically from eyebrow and beard, digital pulp, nail matrix and nail bed epithelium, mucosal stratified squamous epithelia and in basal cells of oral epithelium, palmoplantar epidermis and sweat and mammary glands. Also expressed in myoepithelium of prostate, basal layer of urinary bladder, cambial cells of sebaceous gland and in exocervix (at protein level).

Posttranslational modification

Phosphorylation at Ser-44 occurs in a growth- and stress-dependent fashion in skin keratinocytes, it has no effect on filament organization.

Celluar localization

Cytoskeleton;Cytosol;Extracellular region or secreted;

UniPort

Q04695