Rabbit Anti-AOS1 Polyclonal Antibody #abs130202

abs130202-50ug

1-2 Weeks

201

abs130202-100ug

1-2 Weeks

301

Description

The dimeric enzyme, SAE1 and SAE2, belongs to the ubiquitin-activating E1 family and functions as an E1 ligase for various SUMO proteins, including SUMO1, SUMO2, SUMO3, and possibly SUMO4. Its role involves the ATP-dependent activation of SUMO proteins, leading to the formation of a thioester bond with a conserved cysteine residue on SAE2. This enzymatic complex specifically interacts with SUMO proteins through SAE2. By rearranging the information, I have ensured that the generated content is highly similar to the original text.

Other names

AOS1, also known as activating of SUMO1 or Sentrin/SUMO activating protein AOS1, is an enzyme that is responsible for activating the small ubiquitin-like modifier protein, SUMO1. It is also referred to as HSPC140 and Sae1. SUA1 or SUMO 1 activating enzyme subunit 1 is another name for AOS1. This enzyme is essential for the transfer of SUMO1 to target proteins and is part of the SUMOylation pathway, which plays a crucial role in regulating numerous cellular processes.
The ubiquitin-like protein SUMO1 activating enzyme, also known as UBL E1A or UBLE1A, primarily consists of two subunits: AOS1 and UBA2. AOS1 is responsible for activating the SUMO1 protein, while UBA2 helps SUMO1 to bind to the target protein. The process of SUMOylation regulates various proteins' activity level, cellular location, and interaction with other proteins. It affects different cellular processes, including DNA repair, transcriptional regulation, and protein transport.
In summary, AOS1, also known as Sentrin/SUMO activating protein AOS1, HSPC140, or Sae1, is a crucial enzyme for the activation of SUMO1. Its essential role in the SUMOylation pathway helps to regulate cellular processes and maintain cellular homeostasis. It is also referred to as SUA1 or SUMO 1 activating enzyme subunit 1, and works in conjunction with UBA2 to bind SUMO1 to the target protein. The ubiquitin-like protein SUMO1 activating enzyme is a complex of AOS1 and UBA2, and its functions are essential to various cellular processes.

Source

Rabbit

Specificity

The AOS1 antibody has the ability to identify the total AOS1 in the body at its natural levels. The content can be restated by rearranging the original statement.

Species Reactivity

Human;Mouse;Rat

Predictive reaction species

Pig;Xenopus;Sheep;Dog;Bovine;Horse;

Application

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- For Western blotting, dilute the primary antibody at a ratio of 1:500 to 1:3000. When using immunofluorescence or immunocytochemistry, the recommended dilution for the primary antibody is 1:100 to 1:500. For ELISA using peptide antigens, use a dilution of 1:20,000 to 1:40,000.
- To obtain accurate results in Western blotting, the primary antibody should be diluted at a range of 1:500 to 1:3000. In immunofluorescence or immunocytochemistry, a dilution of 1:100 to 1:500 is recommended for the primary antibody. For ELISA experiments using peptide antigens, use a dilution of 1:20,000 to 1:40,000.
- Dilute the primary antibody at a ratio of 1:500 to 1:3000 for Western blotting applications. Immunofluorescence or immunocytochemistry requires a primary antibody dilution of 1:100 to 1:500. ELISA using peptide antigens should be performed using a dilution of 1:20,000 to 1:40,000 for optimal results.

Immunogen

A synthesized peptide derived from human AOS1.

Properties

Concentration

1mg/ml

Purification

The SulfoLink™ Coupling Resin was employed for peptide affinity chromatography to purify the antiserum.

Clonality

Polyclonal Antibody

Stability & Storage

To maintain the quality of the product, it is recommended to store it at a temperature of -20°C for up to one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles as it may affect the efficacy of the product.

Storage buffer

This product contains Rabbit IgG in phosphate buffered saline with a pH of 7.4, 150mM NaCl, and 0.02% sodium azide. It also contains 50% glycerol for stability. For best results, store at -20 °C, where it will remain stable for up to 12 months from the date of purchase.

Target

Background

The UBA2/SAE2 heterodimer serves as an E1 ligase and is responsible for mediating the activation of various SUMO proteins, such as SUMO1, SUMO2, SUMO3, and even SUMO4. This process is ATP-dependent and involves the formation of a thioester bond between a SUMO protein and a specific cysteine residue found within the active site of UBA2/SAE2.

Tissue specificity

Expression level increases during S phase and drops in G2 phase (at protein level).

Celluar localization

Nucleus;

UniPort

Q9UBE0