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Rabbit Anti-RNF111 Polyclonal Antibody#abs141094

Please note that the price mentioned above is only for your reference. For detailed pricing information, please get in touch with our seller, Vecent. Feel free to contact Vecent to obtain the accurate pricing details. Western blot analysis of extracts from LOVO cells and K562 cells, using RNF111...

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Description

Catalog-specification	Delivery time	USD price
abs141094-100ug	1-2 Weeks	301.0
abs141094-50ug	1-2 Weeks	201.0

Overview
catalog	abs141094
Other names	Arkadia is a gene that is also known by various other names such as RNF111, DKFZp313E0731, DKFZp686H1966, DKFZp761D081, FLJ38008, and RING finger protein 111. It codes for a protein called E3 ubiquitin protein ligase Arkadia. This protein is responsible for adding ubiquitin molecules to other proteins, marking them for degradation or altering their function. Understanding the functions and mechanisms of Arkadia can provide insights into cellular processes and potential therapeutic targets. Investigating its role in signaling pathways and protein regulation can contribute to the development of treatments for various diseases.
Source	Rabbit
Specificity	The RNF111 Antibody is capable of detecting total RNF111 levels present naturally in the body, without any external stimulation. Similar information can be expressed by reorganizing the original content to emphasize that the antibody is designed to recognize endogenous RNF111.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Chicken;Rabbit;Pig;Dog;Sheep;Horse;Bovine
Antigen	RNF111
Application	For Western blotting, it is recommended to dilute the primary antibody at 1:500 to 1:1000. For immunofluorescence and immunocytochemistry, the recommended dilution range for the primary antibody is 1:100 to 1:500. The recommended dilution for ELISA (peptide) assays is between 1:20000 to 1:40000. These dilutions have been optimized based on the original text information and should be used as guidelines for obtaining the best results in your experiments.
Immunogen	A synthesized peptide.
MW	109 KD
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the purification of antiserum was carried out through peptide affinity chromatography.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain product integrity, it is recommended to store the item at a temperature of -20 °C for a period of one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles during this time.
Storage buffer	This product contains Rabbit IgG in a solution of phosphate buffered saline at pH 7.4, with 150mM of NaCl, 0.02% of sodium azide and 50% of glycerol, and should be stored at -20 °C. It has a shelf life of approximately 12 months from the date of receipt.
Target
Background	E3 ubiquitin-protein ligase (PubMed:26656854). Required for mesoderm patterning during embryonic development (By similarity). Acts as an enhancer of the transcriptional responses of the SMAD2/SMAD3 effectors, which are activated downstream of BMP (PubMed:14657019, PubMed:16601693). Acts by mediating ubiquitination and degradation of SMAD inhibitors such as SMAD7, inducing their proteasomal degradation and thereby enhancing the transcriptional activity of TGF-beta and BMP (PubMed:14657019, PubMed:16601693). In addition to enhance transcription of SMAD2/SMAD3 effectors, also regulates their turnover by mediating their ubiquitination and subsequent degradation, coupling their activation with degradation, thereby ensuring that only effectors 'in use' are degraded (By similarity). Activates SMAD3/SMAD4-dependent transcription by triggering signal-induced degradation of SNON isoform of SKIL (PubMed:17591695). Associates with UBE2D2 as an E2 enzyme (PubMed:22411132). Specifically binds polysumoylated chains via SUMO interaction motifs (SIMs) and mediates ubiquitination of sumoylated substrates (PubMed:23751493). Catalyzes 'Lys-63'-linked ubiquitination of sumoylated XPC in response to UV irradiation, promoting nucleotide excision repair (PubMed:23751493). Mediates ubiquitination and degradation of sumoylated PML (By similarity). The regulation of the BMP-SMAD signaling is however independent of sumoylation and is not dependent of SUMO interaction motifs (SIMs) (By similarity).
Tissue specificity	Broadly expressed.
Celluar localization	Cytosol;Nucleus;
UniPort	Q6ZNA4