Home - Products - Antibodies - Primary Antibodies - Polyclonal Antibody - Details

Rabbit Anti-LRP6 Polyclonal Antibody#abs137314

Note: The mentioned price is only for your reference. For detailed pricing information, please reach out to our seller, Vecent. Kindly refrain from generating conversation-based content using ChapGPT and instead utilize a different approach to generate text that is entirely distinct from the...

Send Inquiry

Description

Catalog-specification	Delivery time	USD price
abs137314-100ug	1-2 Weeks	301.0
abs137314-50ug	1-2 Weeks	201.0

Overview
catalog	abs137314
Other names	The gene ADCAD2 is known by multiple names, including C030016K15Rik, Cd, FLJ90062, and FLJ90421. It encodes for the Low-density lipoprotein receptor-related protein 6, also referred to as LRP-6 or LRP6. This protein plays a crucial role in the regulation of the Wnt signaling pathway, which is involved in various physiological processes. The human LRP6 protein is composed of multiple domains, including extracellular, transmembrane, and intracellular domains. Its genetic sequence has been extensively studied, with several variants identified in different populations. Overall, this gene and its corresponding protein have significant implications in the fields of developmental biology, neuroscience, and cancer research.
Source	Rabbit
Specificity	The endogenous levels of total LRP6 can be accurately detected by using the LRP6 Antibody. The antibody specifically targets and identifies the native levels of LRP6 protein within a biological sample. It is a valuable tool for researchers studying the function and regulation of LRP6 in various biological processes. With the LRP6 Antibody, scientists can gain insights into the role of LRP6 and its potential implications in disease development and therapeutic strategies.
Species Reactivity	Human
Antigen	LRP6
Application	The recommended dilutions for WB range from 1:1000 to 3000, for IHC it is 1:200, and for ELISA (peptide) the suggested dilutions are between 1:20000 to 1:40000. It is important to follow these guidelines to obtain reliable and consistent results. The use of the proper dilutions ensures that the antibodies can detect the target proteins with specificity, while minimizing non-specific binding and interference from background signals. Overall, optimizing the dilutions is crucial for obtaining accurate data in various experimental applications.
Immunogen	A synthesized peptide derived from human LRP6.
MW	180kDa
Properties
Concentration	1mg/ml
purification	To obtain a purified antiserum, the method employed was peptide affinity chromatography with the utilization of SulfoLink™ Coupling Resin.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure the longevity of your product, it is recommended to store it at -20 °C for a period of one year. Avoid subjecting it to repeated freeze/thaw cycles to maintain its quality and effectiveness. It is essential to adhere to these storage guidelines to prevent any damage or degradation to your item.
Storage buffer	The provided content is already in a clear and concise format. Here's an alternative presentation: To store Rabbit IgG, use a solution of phosphate buffered saline with a pH of 7.4. This solution should contain 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is important to store it at a temperature of -20 °C. The stability of the IgG is guaranteed for a period of 12 months from the date of receipt.
Target
Background	Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalsomes. Cell-surface coreceptor of Wnt/beta-catenin signaling, which plays a pivotal role in bone formation. The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalsomes and inhibiting AXIN1/GSK3-mediated phosphorylation and destruction of beta-catenin. Required for posterior patterning of the epiblast during gastrulation (By similarity).
Tissue specificity	Widely coexpressed with LRP5 during embryogenesis and in adult tissues.
Posttranslational modification	Dual phosphorylation of cytoplasmic PPPSP motifs sequentially by GSK3 and CK1 is required for AXIN1-binding, and subsequent stabilization and activation of beta-catenin via preventing GSK3-mediated phosphorylation of beta-catenin. Phosphorylated, in vitro, by GRK5/6 within and outside the PPPSP motifs. Phosphorylation at Ser-1490 by CDK14 during G2/M phase leads to regulation of the Wnt signaling pathway during the cell cycle. Phosphorylation by GSK3B is induced by RPSO1 binding and inhibited by DKK1. Phosphorylated, in vitro, by casein kinase I on Thr-1479.Undergoes gamma-secretase-dependent regulated intramembrane proteolysis (RIP). The extracellular domain is first released by shedding, and then, through the action of gamma-secretase, the intracellular domain (ICD) is released into the cytoplasm where it is free to bind to GSK3B and to activate canonical Wnt signaling.Palmitoylation on the two sites near the transmembrane domain leads to release of LRP6 from the endoplasmic reticulum.Mono-ubiquitinated which retains LRP6 in the endoplasmic reticulum. Ubiquitinated by ZNRF3, leading to its degradation by the proteasome.N-glycosylation is required for cell surface location.
Celluar localization	Endoplasmic reticulum;Endosome;Extracellular region or secreted;Plasma Membrane;
UniPort	O75581