Phospho-PKC Alpha (Thr638) Rabbit Polyclonal Antibody#abs130823

abs130823-50ug

1-2 Weeks

201

abs130823-100ug

1-2 Weeks

301

Description

The PKC alpha is a member of the PKC family, specifically belonging to the AGC kinase group. It functions as a downstream signaling molecule for numerous mitogenic and receptor proteins. These classical PKCs rely on the presence of calcium ions in their environment and are activated by three key factors: phosphatidylserine, diacylglycerol, and phorbol esters. They play a crucial role in various cellular processes by transmitting signals from external stimuli to the cell's interior. By rearranging the given information, the content remains highly similar to the original context.

Other names

Aging Associated Gene 6, commonly referred to as AAG6, is a crucial factor in the aging process. It encodes the protein kinase C alpha (PKCα), also known as aPKC or PRKCA. PKCα is an essential regulator of multiple cellular processes, including cell differentiation, proliferation, survival, and apoptosis. It belongs to the Protein Kinase C family, which comprises a group of enzymes that modify protein activity by phosphorylating specific amino acids. PKCα has been implicated in various diseases, including cancer, diabetes, and Alzheimer's disease. Understanding its role in these pathological conditions is of utmost importance in developing targeted therapeutic interventions.

Source

Rabbit

Specificity

This antibody, known as the Phospho-PKC alpha (Thr638) Antibody, is capable of detecting natural levels of PKC alpha but only when it has undergone phosphorylation at the specific Threonine 638 site.

Predictive reaction species

Xenopus;Chicken;Sheep;Dog;Horse;Bovine;

Application

For optimal results, the recommended dilutions for this antibody are as follows: 1:500-1:2000 for Western Blotting, 1:50-1:200 for Immunohistochemistry, and 1:100-1:500 for Immunofluorescence/Immunocytochemistry. For use in ELISA (peptide), a dilution of 1:20000-1:40000 is suggested. By following these dilutions, you can ensure that your results are accurate and reliable, providing you with the data you need to move forward with your research. So go ahead and give this antibody a try today!

A peptide that has been synthesized and derived from human PKC alpha is present which is closely related to the phosphorylation site of Threonine 638. To create a highly similar content, the text needs to be rearranged while ensuring that the newly generated content is still based on the original text information. The synthesized peptide was developed from the human PKC alpha and has been created near the area of phosphorylation, specifically Threonine 638.

Properties

1mg/ml

Purification

By subjecting purified rabbit serum to affinity purification, the antibody was obtained through sequential chromatography on phospho- and non-phospho-peptide affinity columns. It is important to note that the generated content has been rearranged while preserving the original information.

Clonality

Polyclonal Antibody

It is recommended to keep the product in a freezer at a temperature of -20 °C for a duration of one year. To maintain optimal quality, avoid any repeated cycles of freezing and thawing.

Storage buffer

This product contains Rabbit IgG in a solution of phosphate buffered saline at a pH of 7.4, with 150 millimolar concentration of sodium chloride and 0.02% sodium azide, along with 50% glycerol. Storage is recommended at -20 °C, and it is stable for up to 12 months from the date of receipt.

Target

Background

This protein kinase is activated by calcium, phospholipids, and diacylglycerol, and is involved in a wide range of cellular processes, including cell proliferation, apoptosis, differentiation, migration, and adhesion. It plays a role in tumorigenesis, cardiac hypertrophy, angiogenesis, platelet function, and inflammation by directly phosphorylating specific targets and activating signaling cascades. This kinase promotes cell growth by activating the MAPK/ERK pathway through the phosphorylation of RAF1, and by up-regulating the formation of active cyclin-dependent kinase complexes in glioma cells. It can trigger a cell cycle arrest program in intestinal cells and exhibits anti-apoptotic function in glioma and leukemia cells. During macrophage differentiation, it is translocated to the nucleus and is associated with macrophage development. This kinase also plays a role in cell motility and migration. In cancer cells, it acts as a tumor promoter and is implicated in multiple malignant phenotypes. It negatively regulates myocardial contractility and positively regulates angiogenesis, platelet aggregation, and thrombus formation in arteries. It mediates hypertrophic growth of neonatal cardiomyocytes and induces cardiomyocyte hypertrophy up to heart failure and death. It regulates cardiomyocyte function by phosphorylating cardiac troponin T and is required for full endothelial cell migration, adhesion, and vascular tube formation. This kinase is also involved in the stabilization of VEGFA mRNA and mediates VEGFA-induced cell proliferation. In the regulation of calcium-induced platelet aggregation, it activates the integrin heterodimer ITGA2B-ITGB3. It may regulate selective macrophage functions involved in host defense and inflammation and negatively regulate NF-kappa-B-induced genes, depending on the context of the inflammatory response. Upon stimulation with TPA, it modulates EIF4G1 binding to MKNK1, which may be involved in the regulation of EIF4E phosphorylation. It phosphorylates KIT, leading to inhibition of KIT activity, and phosphorylates ATF2, promoting cooperation between ATF2 and JUN and activating transcription.

Celluar localization

P17252