Rabbit Anti-MARK4 Polyclonal Antibody #abs130518

abs130518-50ug

1-2 Weeks

201

abs130518-100ug

1-2 Weeks

301

Description

One of the members of the CAMKL protein kinase family is MARK4, which is typically found in neural progenitors. In cases of gliomas, however, it is often re-expressed.

Other names

FLJ90097 is also known as KIAA1860 and is a gene encoding for microtubule affinity regulating kinase 4. It is also referred to as MAP / microtubule affinity regulating kinase 4L. Another variant of this gene is called MAP / microtubule affinity regulating kinase like 1. Additionally, it is sometimes called MAP/microtubule affinity regulating kinase 4 or MAP/microtubule affinity regulating kinase like 1. It is also commonly known as MARK 4 or MARK 4L, and MARK L1. The protein encoded by this gene is a serine/threonine protein kinase known as MARK4. Another name for this protein is MARK4 serine / threonine protein kinase or MARK4 serine/threonine protein kinase. It is sometimes referred to as MARK4_HUMAN or MARK4S. Another name for this protein is MARKL 1 or MARKL1. Additionally, it is associated with the putative protein product of Nbla00650.

Source

Rabbit

Specificity

MARK4 Antibody detects endogenous levels of MARK4.

Species Reactivity

Human;Mouse

Predictive reaction species

Pig;Sheep;Dog;Horse;Bovine

Application

IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Immunogen

A synthesized peptide derived from human MARK4.

Properties

Concentration

1mg/ml

Purification

The SulfoLink™ Coupling Resin was employed to extract the antiserum through peptide affinity chromatography purification.

Clonality

Polyclonal Antibody

Stability & Storage

-20°C，，。

Storage buffer

This solution contains Rabbit IgG in phosphate buffered saline with a pH of 7.4, a salt concentration of 150mM NaCl, and 0.02% sodium azide, and is also composed of 50% glycerol. To maintain the integrity of the solution, it should be stored at a temperature of -20°C. This solution is safe to use for a year from the date of receipt.

Target

Background

This protein kinase, known as a serine/threonine-protein kinase, has been found to be involved in a range of cellular activities including regulation of the microtubule network, cell cycle progression and energy homeostasis. It is responsible for phosphorylating a number of microtubule-associated proteins, including MAPT, MAP2 and MAP4, which results in the reorganization of microtubules into bundles. Additionally, it is required for the initiation of axoneme extension during cilium assembly. This protein kinase also plays a role in regulating ODF2 localization and phosphorylates the protein in vitro. Along with these cellular functions, the protein kinase can have a negative effect on neuronal cell survival and promote adipogenesis by activating JNK1. However, it can also trigger apoptosis by activating the JNK1 pathway. Interestingly, it has been found to act as a negative regulator of the mTORC1 complex by phosphorylating RPTOR and disrupting the interaction between phosphorylated RPTOR and the RRAGA/RRAGC heterodimer, which is required for mTORC1 activation.

Tissue specificity

Ubiquitous. Isoform 2 is brain-specific (PubMed:11326310). Expressed at highest levels in brain and testis. Also expressed in heart, lung, liver, muscle, kidney and spleen (PubMed:14594945).

Posttranslational modification

Ubiquitinated with 'Lys-29'- and 'Lys-33'-linked polyubiquitins which appear to impede LKB1-mediated phosphorylation. Deubiquitinated by USP9X.Phosphorylated at Thr-214 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39 (PubMed:14976552). Phosphorylated throughout the cell cycle (PubMed:25123532).

Celluar localization

Cytoskeleton;Cytosol;

UniPort

Q96L34