Phospho-PKC-pan (Thr497) Rabbit Polyclonal Antibody#abs130824

abs130824-50ug

1-2 Weeks

201

abs130824-100ug

1-2 Weeks

301

Description

The role of Protein Kinase C (PKC) isoforms as serine/threonine kinases in signal transduction pathways cannot be overstated. These pathways play a crucial part in regulating a vast array of physiological processes. From differentiation and proliferation to gene expression, brain function, membrane transport, and the organization of cytoskeletal and extracellular matrix proteins, PKC isoforms are essential components in the functioning of these pathways. Their utility stretches far and wide, making them an invaluable target for research in the field of medicine and biology.

Other names

Aging associated gene 6, or AAG6, also known as Protein kinase C alpha (PKC-alpha), is an enzyme that plays a key role in cell regulation and signaling pathways. PKC-alpha is a member of the protein kinase C family, which is involved in various cellular processes such as differentiation, proliferation, and apoptosis. Another member of this family is Protein kinase C gamma (PKC-gamma), which is also involved in cell signaling and regulation.
PKC-alpha and PKC-gamma have overlapping yet distinct roles in cellular processes. PKC-alpha is involved in the regulation of cell growth and differentiation, while PKC-gamma is primarily involved in neuronal functions and the regulation of synaptic plasticity. Both isoforms have also been implicated in the pathogenesis of certain diseases. For example, mutations in PKC-gamma have been associated with spinocerebellar ataxia type 14 (SCA14), a neurodegenerative disorder.
Overall, the protein kinase C family of enzymes, including PKC-alpha and PKC-gamma, play important roles in various cellular processes and diseases, and are therefore potential targets for therapeutic intervention.

Source

Rabbit

Specificity

The Phospho-PKC-pan (Thr497) Antibody is designed to specifically recognize and bind to PKC-pan proteins in their phosphorylated state at Threonine 497. This antibody is capable of detecting endogenous levels of PKC-pan when it undergoes phosphorylation at Thr497. By focusing on this specific phosphorylation event, the antibody provides a valuable tool for studying the activation and regulation of PKC-pan in biological processes.

Predictive reaction species

Xenopus;Chicken;Rabbit;Pig;Dog;Bovine;Sheep;Horse;

Application

，：
WB，1:500-1:2000。IHC，1:50-1:200。IF/ICC，1:100-1:500。ELISA（），1:20000-1:40000。。

One potential way to generate similar content based on the original text information could be to focus on the molecular properties of the synthesized peptide and the specific amino acids involved in its structure.
For example:
- The synthesized peptide derived from human PKC-pan was designed to mimic the region surrounding Threonine 497, a crucial phosphorylation site.
- Through careful molecular modeling and chemical synthesis, researchers were able to create a highly stable and effective peptide that can interact with PKC-pan and exert biological effects.
- The peptide contains specific amino acid residues that are critical for its activity, such as serine, glutamic acid, and lysine.
- By targeting PKC-pan and modulating its phosphorylation state, this peptide has the potential to impact various cellular processes such as cell survival, proliferation, and differentiation.
- Further studies are needed to fully elucidate the mechanisms of action for this peptide and explore its potential therapeutic applications.

Properties

1mg/ml

Purification

The affinity-purified antibody was derived from rabbit serum that underwent sequential chromatography on phospho- and non-phospho-peptide affinity columns. Please generate a highly similar content by rearranging the above information while ensuring that the generated content is based on the original text.

Clonality

Polyclonal Antibody

Make sure to store the product at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles.

Storage buffer

This is a description of Rabbit IgG, provided in phosphate buffered saline with a pH of 7.4, supplemented with 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended that this solution is stored at -20 °C and is stable for up to 12 months from the date of receipt.

Target

Background

 Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in positive and negative regulation of cell proliferation, apoptosis, differentiation, migration and adhesion, tumorigenesis, cardiac hypertrophy, angiogenesis, platelet function and inflammation, by directly phosphorylating targets such as RAF1, BCL2, CSPG4, TNNT2/CTNT, or activating signaling cascade involving MAPK1/3 (ERK1/2) and RAP1GAP. Involved in cell proliferation and cell growth arrest by positive and negative regulation of the cell cycle. Can promote cell growth by phosphorylating and activating RAF1, which mediates the activation of the MAPK/ERK signaling cascade, and/or by up-regulating CDKN1A, which facilitates active cyclin-dependent kinase (CDK) complex formation in glioma cells. In intestinal cells stimulated by the phorbol ester PMA, can trigger a cell cycle arrest program which is associated with the accumulation of the hyper-phosphorylated growth-suppressive form of RB1 and induction of the CDK inhibitors CDKN1A and CDKN1B. Exhibits anti-apoptotic function in glioma cells and protects them from apoptosis by suppressing the p53/TP53-mediated activation of IGFBP3, and in leukemia cells mediates anti-apoptotic action by phosphorylating BCL2. During macrophage differentiation induced by macrophage colony-stimulating factor (CSF1), is translocated to the nucleus and is associated with macrophage development. After wounding, translocates from focal contacts to lamellipodia and participates in the modulation of desmosomal adhesion. Plays a role in cell motility by phosphorylating CSPG4, which induces association of CSPG4 with extensive lamellipodia at the cell periphery and polarization of the cell accompanied by increases in cell motility. During chemokine-induced CD4+ T cell migration, phosphorylates CDC42-guanine exchange factor DOCK8 resulting in its dissociation from LRCH1 and the activation of GTPase CDC42 (PubMed:28028151). Is highly expressed in a number of cancer cells where it can act as a tumor promoter and is implicated in malignant phenotypes of several tumors such as gliomas and breast cancers. Negatively regulates myocardial contractility and positively regulates angiogenesis, platelet aggregation and thrombus formation in arteries. Mediates hypertrophic growth of neonatal cardiomyocytes, in part through a MAPK1/3 (ERK1/2)-dependent signaling pathway, and upon PMA treatment, is required to induce cardiomyocyte hypertrophy up to heart failure and death, by increasing protein synthesis, protein-DNA ratio and cell surface area. Regulates cardiomyocyte function by phosphorylating cardiac troponin T (TNNT2/CTNT), which induces significant reduction in actomyosin ATPase activity, myofilament calcium sensitivity and myocardial contractility. In angiogenesis, is required for full endothelial cell migration, adhesion to vitronectin (VTN), and vascular endothelial growth factor A (VEGFA)-dependent regulation of kinase activation and vascular tube formation. Involved in the stabilization of VEGFA mRNA at post-transcriptional level and mediates VEGFA-induced cell proliferation. In the regulation of calcium-induced platelet aggregation, mediates signals from the CD36/GP4 receptor for granule release, and activates the integrin heterodimer ITGA2B-ITGB3 through the RAP1GAP pathway for adhesion. During response to lipopolysaccharides (LPS), may regulate selective LPS-induced macrophage functions involved in host defense and inflammation. But in some inflammatory responses, may negatively regulate NF-kappa-B-induced genes, through IL1A-dependent induction of NF-kappa-B inhibitor alpha (NFKBIA/IKBA). Upon stimulation with 12-O-tetradecanoylphorbol-13-acetate (TPA), phosphorylates EIF4G1, which modulates EIF4G1 binding to MKNK1 and may be involved in the regulation of EIF4E phosphorylation. Phosphorylates KIT, leading to inhibition of KIT activity. Phosphorylates ATF2 which promotes cooperation between ATF2 and JUN, activating transcription.

Celluar localization

P17252/P05771/P05655/P02156/P05129/P24723/P04759