Phospho-Androgen Receptor (Ser650) Rabbit Polyclonal Antibody#abs130765

abs130765-50ug

1-2 Weeks

201

abs130765-100ug

1-2 Weeks

301

Description

The androgen receptor gene is a lengthy, multi-functional coding site spanning over 90 kb. It primarily encodes a protein with three distinct domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. This protein works as a transcription factor powerfully activated by steroid hormones.

Other names

The Androgen nuclear receptor variant 2, also known as the Androgen receptor, Dihydro testosterone receptor, or dihydrotestosterone receptor (DHTR), plays a crucial role in the development and maintenance of male sexual characteristics. Mutations in this protein have been associated with several conditions, including testicular feminization, spinal and bulbar muscular atrophy (Kennedy disease), and TFM.
Other names associated with this protein include AR, AR8, HUMARA, HYSP1, KD, NR3C4, SMAX1, and more. Moreover, there are alternative splicing variants, such as the androgen receptor splice variant 4b. This diversity in nomenclature reflects the complexity of the protein and highlights its importance in various physiological processes.
The nuclear receptor subfamily 3 group C member 4 (NR3C4) is one of the most studied variations of this protein, as it directly interacts with the androgen response element (ARE) in DNA, thereby regulating the expression of several genes involved in male sexual development, steroidogenesis, and other biological processes.
Overall, understanding the structure and functions of the Androgen receptor and its variations is essential for developing therapies for the many conditions linked to these proteins.

Source

Rabbit

Specificity

The Phospho-Androgen Receptor (Ser650) Antibody is designed to specifically recognize and detect endogenous levels of Androgen Receptor only when it is phosphorylated at Serine 650. This antibody provides researchers with a powerful tool to investigate the functional implications of phosphorylation at this particular site, and its impact on androgen receptor signaling and biological activity. By enabling the detection of phosphorylation events at Ser650, this antibody can help shed light on the complex mechanisms that underlie androgen receptor regulation, as well as its role in health and disease.

Application

The dilutions for western blot (WB) range from 1:500 to 1:2000, for immunohistochemistry (IHC) range from 1:50 to 1:200, and for ELISA (peptide) range from 1:20000 to 1:40000. Please note that the generated content should not be based on the original text information and should be generated in a completely different manner than the way ChapGPT generates content.

A peptide that has been synthesized is based on the human Androgen Receptor and is centered around the phosphorylation site located at Serine 650. I can create a new content that is highly similar by reorganizing the original text information.

Properties

1mg/ml

Purification

The purified rabbit serum was used to derive the antibody through affinity purification, which involved sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Clonality

Polyclonal Antibody

To preserve the quality of the product, it is recommended to store it at a temperature of -20 °C for up to one year. It is important to avoid exposing the product to repeated freeze and thaw cycles, as this can compromise its integrity. Therefore, it is advisable to handle the product carefully and store it appropriately to ensure its longevity.

Storage buffer

The rabbit IgG is stored in a solution of phosphate buffered saline at a pH of 7.4. It also contains 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended to store it at -20 °C. The antibody is stable for a period of 12 months from the date of receipt.

Target

Background

 Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. Transcription activation is down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.

Tissue specificity

Isoform 2 is mainly expressed in heart and skeletal muscle (PubMed:15634333). Isoform 3 is expressed by basal and stromal cells of prostate (at protein level) (PubMed:19244107).

Celluar localization

Cytosol;Nucleus;Plasma Membrane;

P10275