Phospho-Myb (Ser532) Rabbit Polyclonal Antibody#abs130764

abs130764-50ug

1-2 Weeks

201

abs130764-100ug

1-2 Weeks

301

Description

Myb, a DNA-binding protein, functions as a transcriptional activator by specifically recognizing the 5'-YAAC[GT]G-3' sequence. Its crucial role lies in regulating the proliferation and differentiation of hematopoietic progenitor cells. Let me generate a distinct version of the content for you based on the information provided.

Other names

The proteins known as C-myb, Cmyb, MYB, and Myb proto oncogene are all closely related and play important roles in cellular processes. Specifically, they are associated with the regulation of genes involved in cell growth, proliferation, and differentiation. MYB belongs to a family of transcription factors that bind to specific DNA sequences and control gene expression.
One specific member of this family is the V-myb myeloblastosis viral oncogene homolog. It is derived from a viral oncogene and has been implicated in the development of leukemia. This oncogene is a result of the integration of the retroviral genome into the host cell DNA, leading to the activation of the MYB gene.
Understanding the functions and mechanisms of these proteins is crucial for studying normal cellular processes as well as disease development. The rearranged content highlights the significance of the C-myb protein family and its involvement in oncogenesis.

Source

Rabbit

Specificity

The Phospho-Myb (Ser532) Antibody specifically recognizes Myb protein levels in cells when it is phosphorylated at serine 532. This antibody is designed to detect the endogenous levels of Myb protein only in its phosphorylated state at serine 532. By targeting this specific phosphorylation site, the antibody allows for precise detection of Myb protein activation and signaling pathways. It can be utilized in various experiments and research studies to investigate the role of Myb phosphorylation and its functional implications in cellular processes.

Predictive reaction species

Zebrafish;Chicken;Rabbit;Xenopus;Dog;Horse;Bovine;Sheep;Pig

Application

The recommended dilutions for WB (Western Blot) range from 1:500 to 1:2000. For IHC (Immunohistochemistry), the suggested dilutions are 1:50 to 1:200. As for IF/ICC (Immunofluorescence/Immunocytochemistry), the optimal dilutions are 1:100 to 1:500. For ELISA (Enzyme-Linked Immunosorbent Assay) using peptides, the recommended dilutions are 1:20000 to 1:40000.

A peptide that has been synthesized from human Myb, specifically targeting the region containing Serine 532's phosphorylation site, can be used for various applications. The synthesized peptide closely resembles the original Myb protein and is a valuable tool for studying Myb's functions in cells. Its composition has been engineered to mimic the natural phosphorylation of Serine 532 to help investigate the molecular mechanisms that regulate Myb's activity. Overall, the synthesized peptide based on human Myb's phosphorylation site at Serine 532 opens new horizons for researchers to better understand the genetic regulation of essential cellular processes.

Properties

1mg/ml

Purification

The process of obtaining the antibody involved purifying rabbit serum and using sequential chromatography on both phospho and non-phospho peptide affinity columns via affinity purification. This resulted in a highly pure and specific antibody.

Clonality

Polyclonal Antibody

To ensure the effectiveness of the storage, it is recommended to keep the store at a temperature of -20 °C for a period of one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles during this time.

Storage buffer

The provided information pertains to Rabbit IgG stored in a solution of phosphate buffered saline with a pH of 7.4. The solution also contains 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended to store this solution at a temperature of -20 °C. The stability of the solution is guaranteed for a period of 12 months from the date of receipt.

Target

Background

This description is about a transcriptional activator, which is a type of DNA-binding protein. It specifically recognizes and binds to the DNA sequence 5'-YAAC[GT]G-3'. This protein is involved in regulating the proliferation and differentiation of hematopoietic progenitor cells, which are important for the development of blood cells. Its function is crucial in maintaining the balance and control of these cells in the body.

Tissue specificity

Negatively regulated by microRNA-155 (miR-155).

Celluar localization

Nucleus;

P10242