Phospho-Met (Tyr1234) Rabbit Polyclonal Antibody#abs130757

abs130757-50ug

1-2 Weeks

201

abs130757-100ug

1-2 Weeks

301

Description

The MET proto-oncogene produces a protein that acts as a receptor for hepatocyte growth factor. This protein has the ability to add phosphate groups to other molecules via its tyrosine kinase activity. The precursor protein undergoes post-translational processing to form two subunits: alpha and beta. These subunits then join together through a disulfide bond to form the mature receptor.

Other names

The Hepatocyte growth factor receptor, also known as the HGF receptor or HGFR, is encoded by the MET gene. This gene belongs to the Met proto-oncogene tyrosine kinase family, which plays a crucial role in cell proliferation, differentiation, and survival. The Met proto-oncogene (hepatocyte growth factor receptor) is also referred to as the scatter factor receptor or SF receptor.
The MET gene is located on chromosome 7 and encodes a protein of approximately 190 kDa. The Met protein is a transmembrane protein composed of an extracellular domain, a single transmembrane domain, and a cytoplasmic domain, which contains the tyrosine kinase activity.
The activation of the Met receptor is triggered by the binding of its ligand, the hepatocyte growth factor (HGF). The binding of HGF to Met induces receptor dimerization and autophosphorylation, leading to the activation of downstream signaling pathways, such as the MAPK/ERK and PI3K/Akt pathways.
Deregulation of Met signaling has been implicated in several human diseases, including cancer, fibrosis, and inflammatory disorders. Thus, inhibitors of Met kinase activity are currently being developed as potential therapeutic agents for cancer and other Met-related diseases.
In conclusion, the HGF receptor or Met proto-oncogene tyrosine kinase is a crucial signaling molecule that regulates diverse cellular functions, and its dysregulation has been implicated in various human diseases. Thus, understanding the mechanisms of Met signaling may lead to the development of novel and effective therapeutics for Met-associated diseases.

Source

Rabbit

Specificity

The Phospho-Met (Tyr1234) Antibody is specifically designed to recognize and bind to Met protein that has undergone phosphorylation specifically at Tyrosine 1234. This antibody allows for the detection of endogenous levels of Met that have been phosphorylated at this particular site. By targeting this phosphorylated form of Met, the antibody enables researchers to study the biological functions and signaling pathways associated with this specific modification.

Predictive reaction species

Rabbit;Dog;Horse

Application

The suggested dilution range for WB is 1:500 to 1:2000. For IF/ICC, the recommended dilution is 1:100 to 1:500. On the other hand, the ideal dilution for ELISA (peptide) is 1:20000 to 1:40000. It is important to follow these recommended dilution ranges to achieve accurate and reliable results.

Human Met-derived peptide, designed around the phosphorylation site of Tyrosine 1234, has been successfully synthesized. The synthesized peptide closely resembles the original human Met sequence and encompasses the essential phosphorylation site at Tyrosine 1234.

Properties

1mg/ml

Purification

Sequential chromatography using phospho- and non-phospho-peptide affinity columns was employed to purify the antibody from rabbit serum. The purification process involved affinity purification of the serum to obtain a highly specific antibody.

Clonality

Polyclonal Antibody

To preserve the product's quality, it is recommended to store it at a temperature of -20 °C for a duration of one year. It is crucial to avoid subjecting the product to repeated freezing and thawing cycles.

Storage buffer

The storage conditions for Rabbit IgG include keeping it in a solution of phosphate buffered saline with a pH of 7.4. Additionally, it should be mixed with 150mM NaCl, 0.02% sodium azide, and 50% glycerol. This mixture should be stored at a temperature of -20 °C. The Rabbit IgG is expected to remain stable for a period of 12 months from the date it is received.

Target

Background

 Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of muscles and neuronal precursors, angiogenesis and kidney formation. In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Promotes also differentiation and proliferation of hematopoietic cells. May regulate cortical bone osteogenesis (By similarity).

Tissue specificity

Expressed in normal hepatocytes as well as in epithelial cells lining the stomach, the small and the large intestine. Found also in basal keratinocytes of esophagus and skin. High levels are found in liver, gastrointestinal tract, thyroid and kidney. Also present in the brain. Expressed in metaphyseal bone (at protein level) (PubMed:26637977).

Celluar localization

Extracellular region or secreted;Plasma Membrane;

P08581