Rabbit Anti-PTTG1 Polyclonal Antibody #abs130289

abs130289-50ug

1-2 Weeks

201

abs130289-100ug

1-2 Weeks

301

Description

Securin is a crucial regulatory protein involved in maintaining chromosome stability, DNA repair and the p53/TP53 pathway. Its mechanism of action involves inhibiting the key proteins responsible for certain cell processes. Specifically, it inhibits Separase/ESPL1 function during mitosis, which helps prevent the degradation of the cohesin complex and subsequent improper chromosome segregation. When anaphase commences, securin undergoes ubiquitination leading to its destruction, and the subsequent release of ESPL1. Interestingly, securin has a dual function as it is required for the activation of ESPL1. Additionally, securin can negatively regulate the transcriptional activity and apoptosis activity of TP53.

Other names

PTTG1, also known as pituitary tumor transforming protein 1, is a protein associated with ESP1 and plays a role in cell division regulation. It is also known as securin and TUTR1. PTTG1 is encoded by the PTTG1 gene and is involved in tumorigenesis. It has multiple isoforms, including isoform CRA_a and isoform CRA_b. PTTG1 is also known as Pds1 and is sometimes referred to as tumor transforming protein 1. MGC126883 and MGC138276 are other aliases for PTTG1. It is implicated in a variety of cancers and may be a potential therapeutic target.

Source

Rabbit

Specificity

The PTTG1 antibody can detect the complete amount of PTTG1 present within an organism. The endogenous levels of PTTG1 can be determined using this antibody. To produce similar content, the information provided in the original text has been rearranged without changing the context.

Species Reactivity

Human

Application

To achieve the best results with our product, we recommend diluting it at a ratio of 1:500 to 1:3000 for Western blotting. For immunofluorescence and immunocytochemistry, a dilution ratio of 1:100 to 1:500 is suggested. If you plan to use ELISA with peptides, we recommend diluting at a ratio of 1:20000 to 1:40000. It's important to follow these guidelines closely to ensure accurate and reliable experimental data.

Immunogen

A synthesized peptide derived from human PTTG1

Properties

Concentration

1mg/ml

Purification

Affinity-chromatography

Clonality

Polyclonal Antibody

Stability & Storage

To maintain the quality of the product, it is recommended to keep it stored at a temperature of -20 °C for a period of one year. It is important to prevent repeated cycles of freezing and thawing, as this can compromise the effectiveness of the product. So, it is a good practice to handle the product with care and ensure proper storage conditions are maintained to avoid any potential damage.

Storage buffer

This is a product description for Rabbit IgG, which is stored in phosphate buffered saline with a pH of 7.4 and contains 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended to store this product at -20°C and it has a shelf life of 12 months from the date of receipt. Use this information to optimize your research and experimental procedures.

Target

Background

This protein acts as a regulatory factor that is essential for maintaining chromosome stability, DNA repair, and the p53/TP53 pathway. Its primary function is to inhibit the functions of important proteins by blocking their actions. During mitosis, it prevents the proteolysis of the cohesion complex and the subsequent segregation of chromosomes by inhibiting the function of Separase/ESPL1. Once anaphase begins, the protein is ubiquitinated, leading to its destruction and the release of ESPL1. Despite its blocking activity, the protein is also necessary for the activation of ESPL1.
Additionally, this regulatory protein negatively regulates the transcriptional and apoptosis activities of TP53. When overexpressed, it can transform cells strongly due to this negative regulation. The protein may also contribute to DNA repair by interacting with Ku, potentially linking DNA damage-response pathways with sister chromatid separation. Overall, this protein is a critical factor in maintaining genome stability and preventing various diseases such as cancer.

Tissue specificity

Expressed at low level in most tissues, except in adult testis, where it is highly expressed. Overexpressed in many patients suffering from pituitary adenomas, primary epithelial neoplasias, and esophageal cancer.

Posttranslational modification

Phosphorylated at Ser-165 by CDK1 during mitosis.Phosphorylated in vitro by ds-DNA kinase.Ubiquitinated through 'Lys-11' linkage of ubiquitin moieties by the anaphase promoting complex (APC) at the onset of anaphase, conducting to its degradation. 'Lys-11'-linked ubiquitination is mediated by the E2 ligase UBE2C/UBCH10.

Celluar localization

Cytosol;Nucleus;

UniPort

O95997