Phospho-Tyrosine Hydroxylase (Ser19) Rabbit Polyclonal Antibody#abs130740

abs130740-50ug

1-2 Weeks

201

abs130740-100ug

1-2 Weeks

301

Description

The conversion of phenylalanine to dopamine is facilitated by tyrosine hydroxylase (EC 1.14.16.2). This enzyme plays a crucial role in the functioning of adrenergic neurons as it acts as the rate-limiting enzyme in catecholamine synthesis. Its involvement in this process highlights the physiological significance of tyrosine hydroxylase.

Other names

DYT14, also known as DYT5b, is a genetic disorder that affects movement. It is caused by mutations in the TH gene, which encodes the enzyme tyrosine hydroxylase. This enzyme is involved in the production of dopamine, a neurotransmitter that plays a key role in movement control. People with DYT14 may experience involuntary muscle contractions and cramping, as well as abnormal posture and gait. The severity of symptoms can vary widely. Treatment may include medications that help to increase dopamine levels in the brain, or, in severe cases, surgery to implant a deep brain stimulator.

Source

Rabbit

Specificity

The antibody known as Phospho-Tyrosine Hydroxylase (Ser19) can identify Tyrosine Hydroxylase present in the body only when it is phosphorylated at Serine 19. This particular antibody can recognize and measure the levels of Tyrosine Hydroxylase that are naturally occurring within the organism and are phosphorylated at Serine 19.

Application

The recommended dilution range for this antibody is WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, and ELISA(peptide) 1:20000-1:40000. When using this antibody, dilution should be carefully optimized based on the application and the sample type. For best results, it is important to use appropriate positive and negative controls and to follow standard protocols for the chosen method. With its high sensitivity and specificity, this antibody is a valuable tool for a wide range of research applications, from basic science to clinical diagnostics.

A peptide synthesized from the human Tyrosine Hydroxylase with a focus on the Serine 19 phosphorylation site is now available. This peptide is similar to the original sequence and can be used in various applications. Its synthesis has been carefully optimized to ensure high purity and quality. You can now obtain this synthesized peptide for your research needs.

Properties

1mg/ml

Purification

The purified rabbit serum was used to create the antibody, which underwent affinity purification through sequential chromatography on phospho- and non-phospho-peptide affinity columns. A highly similar content could be generated by rearranging this information to say that the antibody was produced using rabbit serum that was purified and then underwent affinity purification via sequential chromatography on both phospho- and non-phospho-peptide affinity columns.

Clonality

Polyclonal Antibody

Please make sure to store the product at a temperature of -20 °C for one year. It is important to avoid subjecting it to repeated freeze and thaw cycles.

Storage buffer

This product comprises Rabbit IgG dissolved in phosphate buffered saline (pH 7.4) with 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be stored at a temperature of -20 °C. The product has a shelf life of 12 months from the date of receipt.

Target

Background

The physiology of adrenergic neurons is greatly influenced by an important factor.

Tissue specificity

Mainly expressed in the brain and adrenal glands.

Celluar localization

P07101