Phospho-eIF4E (Ser209) Rabbit Polyclonal Antibody#abs130738

abs130738-50ug

1-2 Weeks

201

abs130738-100ug

1-2 Weeks

301

Description

The dilution ratios for WB, IHC, IF/ICC, and ELISA(peptide) are as follows: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, and ELISA(peptide) 1:20000-1:40000. These ratios determine the optimal working concentrations for each application. It is important to carefully consider the dilution range to achieve accurate and reliable results.

Other names

eIF 4E is also known as Eukaryotic translation initiation factor 4E, which plays a crucial role in the initiation of protein synthesis. It is a key component of the eIF 4F complex, along with the 25 kDa subunit and other associated factors.
The eIF 4F complex is responsible for binding to the cap structure at the 5' end of messenger RNA (mRNA), allowing for the recruitment of ribosomes and the initiation of translation. eIF 4E specifically recognizes the cap structure and interacts with other initiation factors to facilitate the assembly of the translation initiation complex.
In human cells, eIF 4E is encoded by the EIF4E gene and exists in two isoforms, EIF4E1 and EIF4EL1. These isoforms share high sequence similarity but may have distinct functions or regulation mechanisms.
The mRNA cap-binding protein eIF 4E is essential for efficient translation and is involved in various cellular processes. It interacts with numerous proteins and is implicated in the regulation of gene expression, cell growth, and cell survival.
Overall, eIF 4E is a critical component of the protein synthesis machinery, ensuring the efficient translation of mRNA into functional proteins. Its role in translational control makes it a target for therapeutic interventions in diseases where abnormal translation is implicated, such as cancer.

Source

Rabbit

Specificity

The eIF4E antibody, labeled as Phospho-eIF4E (Ser209) Antibody, is designed to specifically target and detect the levels of endogenous eIF4E protein. It effectively recognizes eIF4E protein only when it is phosphorylated at Serine 209. To clarify, this antibody focuses solely on capturing the phosphorylated form of eIF4E at Serine 209 and does not detect the non-phosphorylated version.

Predictive reaction species

Xenopus;Chicken;Rabbit;Pig;Dog;Bovine;Sheep;Horse;

Application

For Western blot, the recommended dilution range is between 1:500 to 1:2000. The suggested dilution for Immunohistochemistry is between 1:50 to 1:200. For Immunofluorescence/Immunocytochemistry, the dilution range of 1:100 to 1:500 is recommended. Lastly, ELISA (peptide) should be diluted between 1:20000 to 1:40000. Please note that these dilutions are recommended starting points and may require further optimization for individual experiments.

A peptide has been synthesized from the human eIF4E protein, focusing on the region surrounding the phosphorylation site of Serine 209. The resulting peptide shares a high degree of similarity with the original protein sequence and may be useful in further research into the functions and interactions of eIF4E in the human body.

Properties

1mg/ml

Purification

The highly similar content generated by rearranging the original text information is as follows:
Sequential chromatography was used to purify the antibody from purified rabbit serum by affinity purification via phospho- and non-phospho-peptide affinity columns.

Clonality

Polyclonal Antibody

To preserve the quality of the product, it is recommended to store it at a temperature of -20 degrees Celsius for a period of one year. It is important to avoid subjecting the product to repeated cycles of freezing and thawing, to maintain its efficacy and potency.

Storage buffer

The rabbit IgG provided is in a buffer solution with phosphate and saline which has a neutral pH of 7.4 with an addition of 150mM of NaCl and 0.02% of sodium azide. There is also 50% glycerol present and it is advised to store it at -20 °C for a maximum of 12 months from the date of receipt, to maintain its stability.

Target

Background

During the initiation of protein synthesis, a crucial step is the recognition and binding of the mRNA cap containing 7-methylguanosine. This essential process is facilitated by a protein called EIF4E, which induces the unwinding of the secondary structures in the mRNA, thus promoting ribosome binding. EIF4E is also a critical component of the CYFIP1-EIF4E-FMR1 complex, which binds to the mRNA cap and mediates translational repression. Within this complex, EIF4E plays a key role in binding to the mRNA cap, enabling efficient translational regulation.

Celluar localization

Cytosol;Extracellular region or secreted;

P06730