Phospho-Progesterone Receptor (Ser294) Rabbit Polyclonal Antibody#abs130734

abs130734-50ug

1-2 Weeks

201

abs130734-100ug

1-2 Weeks

301

Description

The establishment and maintenance of pregnancy rely heavily on the important functions of progesterone. Progesterone receptor, classified as a member of the steroid receptor superfamily, actively facilitates the physiological impacts exerted by progesterone.

Other names

Nuclear receptor subfamily 3 group C member 3, also known as NR3C3, is a protein that plays a crucial role in cellular processes. It is commonly referred to as PGR or PR, which stands for progesterone receptor. The progesterone receptor has two main forms, PRA and PRB, also known as progestin receptor form A and progestin receptor form B, respectively. These forms exhibit distinct functional properties and are involved in various biological pathways. Through rearranging the above text, the following content is generated, keeping the original information intact:
The protein NR3C3, a member of the nuclear receptor subfamily 3 group C, is essential for cellular functions. It is often called PGR or PR, representing the progesterone receptor. Two major forms of the progesterone receptor exist, namely PRA and PRB, referred to as progestin receptor form A and progestin receptor form B, respectively. Each form demonstrates specific functional characteristics and contributes to diverse biological pathways.

Source

Rabbit

Specificity

The Antibody for Phospho-Progesterone Receptor (Ser294) specifically identifies the levels of phosphorylated Progesterone Receptor at Serine 294 in the body. It is designed to detect the presence of endogenous Progesterone Receptor protein only when it is phosphorylated at this specific serine residue.

Predictive reaction species

Pig

Application

。（Western Blot），1:500-1:2000；（Immunofluorescence/Immunocytochemistry），1:100-1:500；（ELISA），，1:20000-1:40000。。

A peptide has been synthesized from the human Progesterone Receptor, specifically targeting the site of Serine 294 phosphorylation. The composition of this peptide is highly similar to the original sequence found in the receptor, allowing for effective interaction and binding with other molecules involved in this signaling pathway. This synthesis represents an important step in further understanding the complex mechanisms underlying progesterone signaling and its role in various physiological processes.

Properties

1mg/ml

Purification

The purified rabbit serum yielded an antibody that underwent affinity purification through a series of chromatography steps on phospho- and non-phospho-peptide affinity columns. The resulting antibody is highly specific and tailored to its intended target. The affinity purification process ensures that the antibody is highly pure and devoid of any impurities that may interfere with its functionality. Therefore, this antibody is a valuable tool for research and diagnostic applications.

Clonality

Polyclonal Antibody

To maintain the quality of the product, it is recommended to store it at a temperature of -20 °C for up to one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles, as this can have a negative impact on its effectiveness. Therefore, it is crucial to handle the product with care and store it properly to ensure its optimal performance.

Storage buffer

The provided information states that the Rabbit IgG should be stored at -20 °C in a solution consisting of phosphate-buffered saline with a pH of 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is mentioned that the IgG is stable for a period of 12 months after its receipt.
To maintain the Rabbit IgG, it is recommended to store it at a temperature of -20 °C. The solution in which it should be preserved contains phosphate-buffered saline with a pH value of 7.4, along with 150mM NaCl. Additionally, the solution contains 0.02% sodium azide and 50% glycerol. This specific storage condition will keep the Rabbit IgG viable for a duration of 12 months starting from the date it was received.

Target

Background

The regulation of gene expression and cellular proliferation and differentiation in target tissues is closely tied to the steroid hormones and their respective receptors. The progesterone receptor isoform has the ability to function as either a transcriptional activator or repressor, depending on the context in which it is expressed.

Tissue specificity

In reproductive tissues the expression of isoform A and isoform B varies as a consequence of developmental and hormonal status. Isoform A and isoform B are expressed in comparable levels in uterine glandular epithelium during the proliferative phase of the menstrual cycle. Expression of isoform B but not of isoform A persists in the glands during mid-secretory phase. In the stroma, isoform A is the predominant form throughout the cycle. Heterogeneous isoform expression between the glands of the endometrium basalis and functionalis is implying region-specific responses to hormonal stimuli.

Celluar localization

Cytosol;Mitochondrion;Nucleus;

P06401