Rabbit Anti-NCoR1 Polyclonal Antibody #abs130206

abs130206-50ug

1-2 Weeks

201

abs130206-100ug

1-2 Weeks

301

Description

N-CoR1, a crucial protein in genetic programming, exerts its regulatory effect on transcription factors and nuclear receptors. It achieves this by facilitating histone deacetylation and chromatin condensation, ultimately modulating the accessibility of various genes to the transcriptional machinery. Notably, N-CoR1 is involved in myogenesis programming. By associating with the thyroid and retinoic acid receptors, alongside the corepressor complex comprising SIN3A/B and histone deacetylases HDAC1 and HDAC2, it dynamically regulates gene expression in the absence of ligands. Furthermore, N-CoR1 engages with the catalytic domain of HDAC9 to fine-tune its functional interactions.

Other names

Nuclear co-repressor 1 (NCoR), also known as hN CoR or KIAA1047, is a critical protein involved in the regulation of gene expression. It interacts with various nuclear receptors such as retinoid X receptor (RXR) and acts as a corepressor to suppress their transcriptional activity. Another name for NCoR is N CoR or N Cor/SMRT corepressor Rip13. This protein, encoded by the NCOR1 gene in humans, is essential in the modulation of thyroid hormone and retinoic acid receptor signaling.
NCoR, or N-CoR1, plays a crucial role in maintaining proper gene expression by silencing specific genetic programs. It forms complexes with other co-repressor proteins to interact with nuclear receptors and inhibit their transcriptional activation. This mechanism is important for fine-tuning cellular responses to various signals, including hormones and developmental cues.
An alternate designation for NCoR is TRAC1, which stands for thyroid hormone and retinoic acid receptor-associated corepressor 1. The protein TRAC1/RIP13 (Rxrip13) functions cooperatively with NCoR to regulate the activity of thyroid hormone and retinoic acid receptors. Together, these co-repressor proteins participate in complex regulatory networks that govern diverse biological processes.
In summary, NCoR, also referred to as hN CoR, KIAA1047, N Cor/SMRT corepressor Rip13, N-CoR1, or NCOR1, is a crucial nuclear receptor co-repressor involved in transcriptional regulation. Its interactions with different nuclear receptors and co-repressor complexes contribute to the modulation of gene expression and cellular responses to hormones and developmental signals. TRAC1, another name for NCoR, reflects its involvement in thyroid hormone and retinoic acid receptor-associated signaling.

Source

Rabbit

Specificity

The endogenous levels of total NCoR1 can be detected using the NCoR1 antibody.

Species Reactivity

Human;Mouse

Application

For Western blotting, the recommended dilution range for this antibody is between 1:500 and 1:3000. In immunohistochemistry, a dilution range of 1:50 to 1:200 is suggested, while for immunofluorescence and immunocytochemistry, a dilution range of 1:100 to 1:500 is optimal. In ELISA applications using a peptide as the antigen, a dilution range of 1:20000 to 1:40000 is recommended for this antibody. It's important to keep in mind these dilution recommendations to achieve the optimal staining or detection with this antibody.

Immunogen

A synthesized peptide derived from human NCoR1.

Properties

Concentration

1mg/ml

Purification

SulfoLink™ Coupling Resin was utilized to purify the antiserum through peptide affinity chromatography.

Clonality

Polyclonal Antibody

Stability & Storage

To ensure optimal storage, it is recommended to store at a temperature of -20 °C for a duration of one year. Avoid exposing the material to multiple freeze and thaw cycles, as this could compromise its integrity and efficacy. It is important to follow these guidelines to preserve the quality of the product.

Storage buffer

: IgG-20°C，pH7.4，150NaCl，0.02%，50%。，12。

Target

Background

This complex, in association with specific nuclear receptors, functions as a transcriptional repressor by facilitating the process of histone deacetylation and the establishment of repressive chromatin structures. These structures play a role in hindering the accessibility of basal transcription factors to the target DNA sequence. Additionally, it is actively involved in the transcriptional repression mediated by BCL6, contributing to its repressor activity.

Posttranslational modification

Ubiquitinated; mediated by SIAH2 and leading to its subsequent proteasomal degradation.

Celluar localization

Cytoskeleton;Cytosol;Nucleus;

UniPort

O75376