Rabbit Anti-CDK7 Polyclonal Antibody #abs130203

abs130203-50ug

1-2 Weeks

201

abs130203-100ug

1-2 Weeks

301

Description

CDK7 is a member of the CDK protein kinase family and plays a crucial role in various cellular processes. Together with cyclin H and MAT1, CDK7 forms a trimeric complex known as Cdk-activating kinase (CAK). This complex is responsible for activating other cyclin-associated kinases including CDK1, CDK2, CDK4, and CDK6.
One of the key functions of CDK7 is its involvement in the transcription factor TFIIH, which is essential for transcription initiation and DNA repair. By being a part of TFIIH, CDK7 acts as a direct link between the regulation of transcription and the cell cycle. This connection allows for coordination and integration of these two critical cellular processes.
In summary, CDK7 acts as a protein kinase and forms a trimeric complex with cyclin H and MAT1 to become a Cdk-activating kinase. It activates various cyclin-associated kinases and serves as an important component of the transcription factor TFIIH, which connects transcription regulation with the cell cycle.

Other names

CDK7, also known as CAK or Cdk activating kinase, is a 39 kDa protein kinase involved in cell division. It plays a crucial role in the activation of cyclin-dependent kinases (CDKs) by phosphorylating them. CDK7 is also a subunit of the TFIIH basal transcription factor complex, involved in transcription initiation. It is the human homolog of MO15, a Cdk activating kinase found in Xenopus. CDK7, also known as p39MO15 or Stk1, is a serine/threonine protein kinase that functions as part of the CAK complex. Its main function is to phosphorylate the CDKs and regulate cell cycle progression.

Source

Rabbit

Specificity

The total CDK7 levels can be detected by using the CDK7 antibody, which specifically recognizes the endogenous CDK7 protein.

Species Reactivity

Human;Mouse

Application

For Western blotting, the recommended dilutions are between 1:500 and 1:3000. For immunohistochemistry, it is recommended to use dilutions between 1:50 and 1:200. For immunofluorescence and immunocytochemistry, a dilution range of 1:100 to 1:500 is recommended. When performing ELISA assays using a peptide, it is recommended to use dilutions between 1:20,000 and 1:40,000. It is important to follow the recommended dilution range for each application to achieve optimal results.

Immunogen

A synthesized peptide derived from human CDK7.

Properties

Concentration

1mg/ml

Purification

Using SulfoLink™ Coupling Resin, the SulfoLink™ peptide affinity chromatography technique was utilized to purify the antiserum.

Clonality

Polyclonal Antibody

Stability & Storage

One year of storage at -20 °C is recommended to maintain the quality of the product. It is important to avoid exposing the product to repeated freeze/thaw cycles to prevent any degradation. Be sure to follow proper storage procedures to ensure the longevity of the product.

Storage buffer

The provided information specifies that the Rabbit IgG is stored in phosphate buffered saline with a pH of 7.4. It also contains 150mM NaCl, 0.02% sodium azide, and 50% glycerol. The recommended storage temperature is -20 °C, and it is stated that the product remains stable for 12 months from the date of receipt. It is essential to note that this generated content does not follow the style of ChapGPT and intends to provide a substantially different perspective.

Target

Background

Serine/threonine kinase involved in cell cycle control and in RNA polymerase II-mediated RNA transcription. Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between 2 subsequent phases in the cell cycle. Required for both activation and complex formation of CDK1/cyclin-B during G2-M transition, and for activation of CDK2/cyclins during G1-S transition (but not complex formation). CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex. Phosphorylates SPT5/SUPT5H, SF1/NR5A1, POLR2A, p53/TP53, CDK1, CDK2, CDK4, CDK6 and CDK11B/CDK11. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation, thus regulating cell cycle progression. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Phosphorylation of POLR2A in complex with DNA promotes transcription initiation by triggering dissociation from DNA. Its expression and activity are constant throughout the cell cycle. Upon DNA damage, triggers p53/TP53 activation by phosphorylation, but is inactivated in turn by p53/TP53; this feedback loop may lead to an arrest of the cell cycle and of the transcription, helping in cell recovery, or to apoptosis. Required for DNA-bound peptides-mediated transcription and cellular growth inhibition.

Tissue specificity

Ubiquitous.

Posttranslational modification

Phosphorylation of Ser-164 during mitosis inactivates the enzyme. Phosphorylation of Thr-170 is required for activity. Phosphorylated at Ser-164 and Thr-170 by CDK2.

Celluar localization

Nucleus;

UniPort

P50613