Rabbit Anti-NYREN18 Polyclonal Antibody #abs130208

abs130208-50ug

1-2 Weeks

201

abs130208-100ug

1-2 Weeks

301

Description

Induced by beta and gamma interferons, NYREN18 acts as a specific down-regulator of the NEDD8 conjugation system. It plays a crucial role in recruiting NEDD8 and its conjugates to the proteasome for degradation. Among its isoforms, isoform 1 exhibits higher efficiency in promoting the degradation of NEDD8 compared to isoform 2. Remarkably, NYREN18 directly interacts with NEDD8 and PSMD4/S5a, a regulatory subunit of the 26S proteasome. This interaction facilitates the efficient degradation process and contributes to the regulation of intracellular NEDD8 levels.

Other names

NUB1, also known as NEDD8 ultimate buster 1 or negative regulator of ubiquitin-like proteins 1, is a protein that plays a crucial role in regulating the activity of ubiquitin-like proteins in the cell. It is also known by the aliases NUB1L, NY REN 18, or NYREN18, and belongs to the NUB1 family of proteins.
As a negative regulator of ubiquitin-like proteins, NUB1 acts to prevent the inappropriate activation of these proteins and their downstream pathways. This makes it a critical component in the maintenance of proper cellular function and homeostasis.
Additionally, NUB1 has been implicated in the development and progression of renal cell carcinoma, as it is expressed at high levels in these tumors. It is also a target for immunotherapy, as it is recognized by the immune system as a tumor-associated antigen.
Overall, the function and role of NUB1 in the cell and in disease make it a promising target for further research and drug development efforts.

Source

Rabbit

Specificity

The NYREN18 antibody is capable of detecting the total amount of NYREN18 protein present in an organism's cells. This detection method works by recognizing endogenous levels of the protein within a given sample. It is important to note that the NYREN18 antibody specifically targets the total protein level of NYREN18, rather than any individual isoform or variant. This detection method is highly effective for researchers looking to analyze NYREN18 protein expression in select cells or tissues.

Species Reactivity

Human;Mouse;Monkey

Application

For Western blotting, dilute the primary antibody to a range of 1:500 to 1:3000. In the case of immunohistochemistry, dilute the antibody to a range of 1:50 to 1:200. For immunofluorescence/immunocytochemistry, a dilution range of 1:100 to 1:500 is recommended. When performing an ELISA assay using a peptide, dilute the antibody to a range of 1:20000 to 1:40000. Remember to adhere to these dilution guidelines to obtain the most accurate and reliable results.

Immunogen

A synthesized peptide derived from human NYREN18.

Properties

Concentration

1mg/ml

Purification

Using SulfoLink™ Coupling Resin, the antiserum underwent purification via peptide affinity chromatography to obtain a highly purified form.

Clonality

Polyclonal Antibody

Stability & Storage

To preserve the quality of the product, it is recommended to store it at a temperature of -20 °C for up to one year. It is important to avoid subjecting the product to repeated freeze and thaw cycles, as this can negatively impact its stability. It is advised to take appropriate precautions to ensure that the storage conditions are maintained consistently and that the product remains in the best possible condition for use.

Storage buffer

The provided information states that Rabbit IgG is stored in phosphate buffered saline, pH 7.4, containing 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be stored at -20 °C and remains stable for 12 months from the date of receipt. Please note that the generated content is based on the original text information and has been rearranged to deliver the same message.

Target

Background

This protein functions as a specific down-regulator of the NEDD8 conjugation system. Its role is to recruit NEDD8, UBD, and their conjugates to the proteasome for effective degradation. Interestingly, isoform 1 has been found to more efficiently promote the degradation of NEDD8 compared to isoform 2. It is a crucial protein that plays a pivotal role in regulating the NEDD8 conjugation system and maintaining cellular homeostasis. Its functional significance cannot be undermined, and its workings have long been a topic of intense research in the scientific community.

Tissue specificity

Isoform 1 is expressed in various tissues, but its lowest expression is observed in the pancreas. On the other hand, isoform 2 is primarily expressed in leukocytes, liver, prostate, and skeletal muscle, although it is also present in other tissues. Overall, both isoforms have distinct expression patterns across different tissues, highlighting their diverse roles in various physiological processes.

Celluar localization

Cytosol;Nucleus;

UniPort

Q9Y5A7