Rabbit Anti-SENP1 Polyclonal Antibody #abs130211

abs130211-50ug

1-2 Weeks

201

abs130211-100ug

1-2 Weeks

301

Description

SENP1 is an important protease in the SUMO pathway, serving two vital roles. Firstly, it processes full-length SUMO1, SUMO2, and SUMO3 into their mature forms, which is crucial in the regulation of several cellular processes. Secondly, it deconjugates SUMO1, SUMO2, and SUMO3 from targeted proteins, which is especially important in the control of protein localization and activity. For instance, SENP1 can deconjugate SUMO1 from HIPK2 and HDAC1, which impairs their functions and activity. Furthermore, SENP1 is known to reduce transcriptional repression activity by deconjugating SUMO1 from HDAC1, indicating its central role in gene regulation. Overall, SENP1 plays an essential part in the SUMO pathway and has significant implications for cellular physiology and function.

Other names

SENP1, also known as Sentrin-specific protease 1, is an important enzyme involved in the regulation of protein modification by SUMO (small ubiquitin-like modifier) molecules. This protease, encoded by the SENP1 gene in humans, plays a crucial role in the removal of SUMO proteins from target proteins through its specific enzymatic activity.
SENP1_HUMAN, another name for SENP1, belongs to the class of Sentrin/SUMO-specific proteases. It specifically acts on SUMO1/sentrin molecules, cleaving them from target proteins and reversing the SUMOylation process. This process of deconjugation mediated by SENP1 is critical for the dynamic regulation of protein function and cellular processes.
Also referred to as Sentrin/SUMO-specific protease SENP1 or Sentrin/SUMO-specific protease 1, SENP1 is responsible for maintaining the balance and fine-tuning of SUMOylation in cells. It is involved in various cellular mechanisms like DNA repair, cell cycle progression, and transcriptional regulation.
SENP1 is often abbreviated as SuPr2 and is considered an essential mediator in the SUMOylation pathway. Its enzymatic activity ensures the timely removal of SUMO proteins from target proteins, allowing for their correct functional modifications.
In summary, SENP1 is a highly regulated protease that specifically acts on SUMO proteins. It plays a vital role in maintaining the balance and dynamics of protein modification processes, making it a crucial player in various cellular activities.

Source

Rabbit

Specificity

The antibody against SENP1 can identify the complete amount of SENP1 present in the body. This antibody can recognize natural levels of SENP1, indicating its effectiveness in detecting the protein in various samples.

Species Reactivity

Human;Mouse

Predictive reaction species

Pig;Sheep;Dog;Bovine;

Application

The recommended dilutions for Western blot (WB) range from 1:500 to 1:3000. For immunohistochemistry (IHC), the suggested dilutions vary from 1:50 to 1:200. If you are performing immunofluorescence (IF) or immunocytochemistry (ICC), it is recommended to dilute the antibody between 1:100 and 1:500. For ELISA assays using peptides, the recommended dilution is between 1:20000 and 1:40000. It is important to follow these dilution guidelines to ensure accurate and reliable results.

Immunogen

A synthesized peptide derived from human SENP1.

Properties

Concentration

1mg/ml

Purification

SulfoLink™ Coupling Resin was employed for peptide affinity chromatography to purify the antiserum.

Clonality

Polyclonal Antibody

Stability & Storage

To ensure the optimal storage of the product, it is recommended to store it at a temperature of -20 °C for a period of one year. It is important to avoid exposing the product to repeated freeze and thaw cycles, as this may impact its stability and efficacy. Thus, always take precautionary measures to protect the product from temperature fluctuations, and store it in a cool and dry place. These guidelines will help maintain the product's quality and ensure its effectiveness over time.

Storage buffer

The Rabbit IgG is preserved in a solution of phosphate buffered saline with a pH of 7.4. It is formulated with 150mM NaCl, 0.02% sodium azide, and 50% glycerol for long-term stability. Keep it stored at -20 °C, where it can stay usable for up to 12 months from the moment of receipt.

Target

Background

A protease is responsible for two crucial functions in the SUMO pathway: the processing of full-length SUMO1, SUMO2, and SUMO3 into their mature forms, and the removal of SUMO1, SUMO2, and SUMO3 from targeted proteins. It specifically deconjugates SUMO1 from HIPK2, HDAC1, and BHLHE40/DEC1, leading to a decrease in their transcriptional repression activity. Additionally, it deconjugates SUMO1 from CLOCK, reducing its transcriptional activation activity. Furthermore, it desumoylates MTA1 by removing SUMO2 from it. Notably, when it desumoylates CCAR2, it diminishes its interaction with SIRT1, as reported in a study (PubMed:25406032). Feel free to rearrange and modify the generated content to best suit your needs.

Tissue specificity

Highly expressed in testis. Expressed at lower levels in thymus, pancreas, spleen, liver, ovary and small intestine.

Celluar localization

Nucleus;

UniPort

Q9P0U3