Rabbit Anti-MEF2C Polyclonal Antibody #abs130338

abs130338-50ug

1-2 Weeks

201

abs130338-100ug

1-2 Weeks

301

Description

MEF2C is a member of the MADS family of transcription factors that specifically binds to the MEF2 element present in the regulatory regions of various muscle-specific genes. It is believed to play a crucial role in myogenesis, neurogenesis, and the development of cortical architecture. The gene encoding MEF2C can produce three splice-variant isoforms with differential expression patterns and functions. Overall, MEF2C is a key regulator of gene expression in muscle cells and brain development.

Other names

MEF2C, also known as myocyte-specific enhancer factor 2C, is a transcription enhancer factor that plays an important role in the development and differentiation of muscles. It belongs to the MADS-box family of transcription factors and is encoded by the C5DELq14.3 gene.
MEF2C is critical for regulating the expression of genes involved in muscle cell proliferation and differentiation. It has been found to be indispensable for the formation of cardiac and skeletal muscles, and mutations in the MEF2C gene have been linked to numerous developmental disorders.
The MEF2C protein contains several conserved domains, including a DNA-binding domain that recognizes specific DNA sequences and a transcription activation domain that recruits other proteins to initiate gene expression. MEF2C also interacts with other transcription factors, coactivators, and corepressors to fine-tune gene expression during muscle development.
In conclusion, MEF2C is a crucial transcription factor that plays a pivotal role in regulating muscle development and differentiation. Understanding its functions and mechanisms of action will provide valuable insights into the molecular basis of numerous developmental disorders that are associated with MEF2C mutations.

Source

Rabbit

Specificity

MEF2C Antibody detects endogenous levels of MEF2C

Species Reactivity

Human;Mouse

Application

IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Immunogen

A synthesized peptide derived from human MEF2C

Properties

Concentration

1mg/ml

Purification

Immunogen affinity purified

Clonality

Polyclonal Antibody

Stability & Storage

To preserve the quality of the product, it is advised to keep it at a temperature of -20 °C for a year. It is important to avoid exposing it to multiple freeze and thaw cycles, as this can have a negative impact on its stability and efficacy. It is recommended to store the product in a cool and dry place to ensure its longevity and effectiveness.

Storage buffer

The recommended storage condition for Rabbit IgG is at -20 °C in phosphate buffered saline with a pH of 7.4. It should be supplemented with 150mM NaCl, 0.02% sodium azide, and 50% glycerol. This formulation ensures stability for 12 months from the date of receipt. Kindly note that the content generated using language models will not follow the original text and is expected to be significantly different in nature.

Target

Background

This transcription activator is known for its ability to bind specifically to the MEF2 element found in various muscle-specific genes' regulatory regions. Its functions include controlling cardiac morphogenesis and myogenesis, participating in vascular development, and playing a crucial role in hippocampal dependent learning and memory. It is also essential for proper neuronal development and electrical activity within the neocortex and necessary for megakaryocyte and platelet development, bone marrow B-lymphopoiesis, and B-cell survival and proliferation in response to BCR stimulation. In addition, this activator may be involved in neurogenesis and the development of cortical architecture. Interestingly, certain isoforms lacking the repressor domain appear to be more active than others.

Tissue specificity

Expressed in brain and skeletal muscle.

Posttranslational modification

Phosphorylation on Ser-59 enhances DNA binding activity (By similarity). Phosphorylation on Ser-396 is required for Lys-391 sumoylation and inhibits transcriptional activity.Acetylated by p300 on several sites in diffentiating myocytes. Acetylation on Lys-4 increases DNA binding and transactivation (By similarity).Sumoylated on Lys-391 with SUMO2 but not by SUMO1 represses transcriptional activity.Proteolytically cleaved in cerebellar granule neurons, probably by caspase 7, following neurotoxicity. Preferentially cleaves the CDK5-mediated hyperphosphorylated form which leads to neuron apoptosis and transcriptional inactivation.

Celluar localization

Cytosol;Nucleus;

UniPort

Q06413