Phospho-Lyn (Tyr507) Rabbit Polyclonal Antibody#abs130747

abs130747-50ug

1-2 Weeks

201

abs130747-100ug

1-2 Weeks

301

Description

Lyn is a member of the Src family of tyrosine kinases and has an important role in signaling through the B cell antigen receptor.

Other names

The protein kinase AA407514 (also known as EC 2.7.10.2), FLJ26625, Hck 2, JTK 8, or JTK8 is a novel tyrosine kinase that is related to Lck and Yes. It is encoded by the LYN gene, which is a proto-oncogene belonging to the Src family of tyrosine kinases. The protein is also named LYN_HUMAN, ONCOGENE LYN, p53Lyn, p56Lyn, Tyrosine protein kinase LYN, Tyrosine-protein kinase Lyn, V yes 1 Yamaguchi sarcoma viral related oncogene homolog, or Yamaguchi sarcoma viral (v yes 1) related oncogene homolog.
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Source

Rabbit

Specificity

The antibody Phospho-Lyn (Tyr508) specifically recognizes the presence of phosphorylated Lyn at Tyrosine 507 in endogenous levels.

Predictive reaction species

Chicken;Rabbit;Zebrafish;Dog;Horse;Bovine;Sheep

Application

WB，1:500-1:2000；IHC，1:50-1:200；IF/ICC，1:100-1:500；ELISA（），1:20000-1:40000。。

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Properties

1mg/ml

Purification

Via sequential chromatography on phospho- and non-phospho-peptide affinity columns, the antibody is obtained from purified rabbit serum by affinity purification.

Clonality

Polyclonal Antibody

To preserve the quality of the product, it is recommended to store it at a temperature of -20 °C for a duration of one year. It is essential to avoid repeated cycles of freezing and thawing to prevent possible damage to the product. It is important to follow these guidelines to ensure the product's effectiveness and longevity.

Storage buffer

The recommended storage conditions for Rabbit IgG are at -20 °C. It is supplied in phosphate buffered saline with a pH of 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol. This formulation provides stability for a period of 12 months from the date of receipt.

Target

Background

This non-receptor tyrosine-protein kinase is responsible for transmitting signals from receptors on the cell surface. It plays a vital role in regulating various immune responses, such as innate and adaptive immunity, hematopoiesis, growth factor and cytokine responses, integrin signaling, and the response to DNA damage and genotoxic agents. Its primary function is as a negative regulator, but it can also act as an activator depending on the situation.
One of its key roles is in initiating and regulating the B-cell response. It is crucial for the differentiation, proliferation, survival, and apoptosis of B-cells, and is also important for maintaining immune self-tolerance. It acts downstream of several immune receptors, including the B-cell receptor, CD79A, CD79B, CD5, CD19, CD22, FCER1, FCGR2, FCGR1A, TLR2, and TLR4. Additionally, it plays a role in the inflammatory response to bacterial lipopolysaccharide.
In hematopoietic progenitors, platelets, erythrocytes, dendritic cells, neutrophils, and eosinophils, this kinase mediates responses to cytokines and growth factors. It is involved in regulating cell proliferation, survival, differentiation, migration, adhesion, degranulation, and cytokine release. By phosphorylating immunoreceptor tyrosine-based inhibitory motifs (ITIM), it down-regulates signaling pathways and modulates signaling through interactions with phosphatases.
This kinase phosphorylates various proteins involved in immune response and cell signaling, including LIME1, BTK, CBL, CD5, CD19, CD72, CD79A, CD79B, CSF2RB, DOK1, HCLS1, LILRB3/PIR-B, MS4A2/FCER1B, PTK2B/PYK2, SYK, TEC, SIRPA, PTPN6/SHP-1, PTPN11/SHP-2, and INPP5D/SHIP-1. It is also responsible for phosphorylating the BCR-ABL fusion protein and FER in response to FCER1 activation. Moreover, it mediates KIT phosphorylation and acts as an effector of EPOR in controlling KIT expression during erythroid differentiation.
Depending on the context, this kinase can activate or inhibit various signaling cascades. It regulates phosphatidylinositol 3-kinase activity, AKT1 activation, and the MAP kinase signaling cascade, including MAP2K1/MEK1, MAPK1/ERK2, MAPK3/ERK1, MAPK8/JNK1, and MAPK9/JNK2. It also mediates the activation of STAT5A and/or STAT5B and the phosphorylation of LPXN on 'Tyr-72'. Additionally, its kinase activity facilitates TLR4-TLR6 heterodimerization and signal initiation.
In summary, this non-receptor tyrosine-protein kinase is a crucial regulator of immune responses, hematopoiesis, growth factor and cytokine signaling, and integrin signaling. It acts as a negative regulator but can also function as an activator depending on the context. Its phosphorylation of various proteins and modulation of signaling pathways play essential roles in cell function and immune response.

Tissue specificity

Detected in monocytes (at protein level). Detected in placenta, and in fetal brain, lung, liver and kidney. Widely expressed in a variety of organs, tissues, and cell types such as epidermoid, hematopoietic, and neuronal cells. Expressed in primary neuroblastoma tumors.

Celluar localization

Cytosol;Extracellular region or secreted;Golgi apparatus;Lysosome;Mitochondrion;Nucleus;Plasma Membrane;

P07948