Phospho-JunB (Ser259) Rabbit Polyclonal Antibody#abs130825

abs130825-50ug

1-2 Weeks

201

abs130825-100ug

1-2 Weeks

301

Other names

Source

Rabbit

Specificity

The Antibody for Phospho-JunB (Ser259) is designed to specifically recognize and measure the presence of phosphorylated JunB protein at Serine 259. It is sensitive enough to detect endogenous levels of JunB only when it is phosphorylated at this specific site.

Predictive reaction species

Pig;Sheep;Dog;Horse;Bovine

Application

The recommended dilutions for using this antibody are as follows: for Western blotting, use a dilution of 1:500 to 1:2000; for immunohistochemistry, use a dilution range of 1:50 to 1:200; for immunoprecipitation, the antibody can be used without any dilution; and for ELISA (with peptide), we suggest a dilution range of 1:20000 to 1:40000. Please note that these dilutions are just a guideline, and the optimal dilution for your experiment may vary depending on your specific needs and conditions. It's always important to perform a titration experiment to determine the best dilution for your particular application.

Human JunB is the source of a synthesized peptide that focuses on the phosphorylation site of Serine 259. To create an analogous content, the information provided in the original text must be maintained while rearranging the structure and phrasing. It is crucial to note that the generated content should vary significantly from the original text and should not follow the conversational approach of ChapGPT.

Properties

1mg/ml

Purification

The purified antibody was obtained from rabbit serum by performing affinity purification through sequential chromatography on affinity columns containing both phospho- and non-phospho-peptides. The resulting product is highly specific for its corresponding target antigen.

Clonality

Polyclonal Antibody

To maintain the quality of this product, it is recommended to store it at a temperature of -20 °C for a period of one year. It is best to avoid subjecting it to repeated cycles of freezing and thawing. Taking such precautions will help to preserve the properties of the product and ensure its longevity. Please prioritize these storage guidelines for the optimal use of the product.

Storage buffer

The rabbit IgG is formulated in a solution of phosphate buffered saline at a pH value of 7.4, containing 150 millimoles of sodium chloride, and with 0.02% of sodium azide and 50% glycerol added to enhance stability. The product should be stored at a temperature of -20 °C for optimal preservation, and it is expected to maintain its efficacy for up to 12 months from the date of purchase.

Target

Background

This transcription factor plays an important role in modulating gene expression after the initial response to growth factors. It specifically recognizes and binds to the DNA sequence 5'-TGA[CG]TCA-3'.

Tissue specificity

By growth factors.

Celluar localization

Nucleus;

P17275