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Rabbit Anti-HIF-2-alpha Polyclonal Antibody#abs133265

Description

Catalog-specification	Delivery time	USD price
abs133265-100ug	1-2 Weeks	301.0
abs133265-50ug	1-2 Weeks	201.0

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Overview
catalog	abs133265
Other names	The Basic helix loop helix PAS protein MOP2, also known as bHLHe73, is a member of the PAS superfamily. It is also called class E basic helix-loop-helix protein 73 or endothelial PAS domain-containing protein 1 (EPAS1). EPAS1, also known as HIF-2-alpha or hypoxia-inducible factor 2 alpha, is essential for the regulation of cellular responses to low oxygen levels. It is highly similar to HIF-1-alpha-like factor (HLF) and HIF2A. EPAS1/HIF-2-alpha is involved in angiogenesis, cell proliferation, and glucose metabolism. It is expressed in various tissues, including endothelial cells, lung, heart, liver, kidney, and brain. It plays a major role in the development and maintenance of the vascular system. The structure of EPAS1/HIF-2-alpha includes a basic helix-loop-helix (bHLH) domain, which is required for DNA binding, and a PAS domain, which mediates protein-protein interactions. EPAS1/HIF-2-alpha regulates gene expression by binding to hypoxia-responsive elements (HREs) in promoter regions of target genes. It interacts with transcriptional co-activators such as p300/CBP, CREB-binding protein (CBP), and SRC-1, and the co-repressors mSin3A and HDAC3. EPAS1/HIF-2-alpha is a potential therapeutic target for cancer and cardiovascular diseases. Pharmacological inhibition of EPAS1/HIF-2-alpha may reduce tumor growth, angiogenesis, and metastasis.
Source	Rabbit
Specificity	The antibody for HIF-2-alpha can effectively recognize the natural levels of total HIF-2-alpha protein present in a biological sample. By using this antibody, researchers can accurately detect and measure the expression of HIF-2-alpha in cells or tissues. The antibody provides a reliable tool for studying the role and regulation of HIF-2-alpha in various physiological and pathological processes.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Pig;Dog;Horse
Antigen	HIF-2-alpha
Application	Based on the original text information, the recommended dilution for Western Blot (WB) is 1:1000-3000, for Immunocytochemistry/Immunofluorescence (ICC/IF) it is 1:1000-3000, and for ELISA (peptide) it is 1:20000-1:40000. These dilutions ensure optimal results for these experimental techniques.
Immunogen	A peptide that is synthesized and derived from the human HIF-2-alpha has been developed and utilized. The newly created peptide has demonstrated a high degree of similarity to the original HIF-2-alpha protein and is utilized in various medical applications. The development of the peptide was based on the previous research and knowledge of the HIF-2-alpha protein structure and functions. The synthesized peptide has garnered significant interest in the scientific community due to its therapeutic potential and has opened up new avenues for further research.
MW	96kDa
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was utilized in the process of peptide affinity chromatography to purify the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBBP and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD.
Tissue specificity	Expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells.
Posttranslational modification	In normoxia, is probably hydroxylated on Pro-405 and Pro-531 by EGLN1/PHD1, EGLN2/PHD2 and/or EGLN3/PHD3. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization (By similarity).In normoxia, is hydroxylated on Asn-847 by HIF1AN thus probably abrogating interaction with CREBBP and EP300 and preventing transcriptional activation.Phosphorylated on multiple sites in the CTAD.The iron and 2-oxoglutarate dependent 3-hydroxylation of asparagine is (S) stereospecific within HIF CTAD domains.
Celluar localization	Cytosol;Nucleus;
UniPort	Q99814