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Rabbit Anti-Histone H2A.Z Polyclonal Antibody#abs133267

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Description

Catalog-specification	Delivery time	USD price
abs133267-100ug	1-2 Weeks	301.0
abs133267-50ug	1-2 Weeks	201.0

Overview
catalog	abs133267
Other names	The H2A histone family is comprised of several members, including H2A histone family member A, H2A histone family member M, H2A.1, H2A.2, H2A/a, H2A/m, H2A1B_HUMAN, H2AFA, H2AFM, HIST1H2AB, HIST1H2AE, Histone 1 H2ab, Histone 1 H2ae, Histone cluster 1 H2ab, Histone cluster 1 H2ae, Histone H2A type 1-B/E, Histone H2A.2, Histone H2A/a, Histone H2A/m, and Histone H2AE. These various members all exhibit slightly different functions and roles within the cell, but they all play important roles in regulating chromatin structure and gene expression. By studying these proteins and their functions, scientists hope to gain a deeper understanding of how the genome is organized and regulated, which could lead to breakthroughs in fields like cancer biology and developmental biology.
Source	Rabbit
Specificity	Our Histone H2A.Z Antibody is capable of detecting the total levels of endogenous Histone H2A.Z. It specifically recognizes the presence of Histone H2A.Z in biological samples.
Species Reactivity	Human;Mouse;Rat
Antigen	Histone H2A.Z
Application	For Western blotting, a dilution of 1:1000-3000 is recommended. When performing immunohistochemistry, a dilution of 1:200 is optimal. For ICC/IF, the same dilution as for IHC is appropriate. When using a peptide-based ELISA, it is advisable to dilute the sample between 1:20000-1:40000 for accurate results. Always follow the recommended dilutions for best results.
Immunogen	Human Histone H2A.Z can be used to synthesize a peptide with high similarity.
MW	13kDa
Properties
Concentration	1mg/ml
purification	Peptide affinity chromatography was employed to purify the antiserum, and SulfoLink™ Coupling Resin was used in the process.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain its quality, it is recommended to store the product at a temperature of -20 °C for a duration of one year. It is important to prevent any freeze/thaw cycles from occurring repeatedly.
Storage buffer	The rabbit IgG comes in a solution of phosphate buffered saline with a pH of 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. It should be stored at -20°C and is stable for 12 months from the date of receipt. It is important to note that the content generated must be based on the original text information but should be written in a different and distinct way that does not follow ChapGPT's approach.
Target
Background	Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Posttranslational modification	Deiminated on Arg-4 in granulocytes upon calcium entry.Monoubiquitination of Lys-120 (H2AK119Ub) by RING1, TRIM27 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. It is involved in the initiation of both imprinted and random X inactivation. Ubiquitinated H2A is enriched in inactive X chromosome chromatin. Ubiquitination of H2A functions downstream of methylation of 'Lys-27' of histone H3 (H3K27me). H2AK119Ub by RNF2/RING2 can also be induced by ultraviolet and may be involved in DNA repair. Monoubiquitination of Lys-120 (H2AK119Ub) by TRIM27 may promote transformation of cells in a number of breast cancers (PubMed:25470042). Following DNA double-strand breaks (DSBs), it is ubiquitinated through 'Lys-63' linkage of ubiquitin moieties by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168, leading to the recruitment of repair proteins to sites of DNA damage. Ubiquitination at Lys-14 and Lys-16 (H2AK13Ub and H2AK15Ub, respectively) in response to DNA damage is initiated by RNF168 that mediates monoubiquitination at these 2 sites, and 'Lys-63'-linked ubiquitin are then conjugated to monoubiquitin; RNF8 is able to extend 'Lys-63'-linked ubiquitin chains in vitro. H2AK119Ub and ionizing radiation-induced 'Lys-63'-linked ubiquitination (H2AK13Ub and H2AK15Ub) are distinct events.Phosphorylation on Ser-2 (H2AS1ph) is enhanced during mitosis. Phosphorylation on Ser-2 by RPS6KA5/MSK1 directly represses transcription. Acetylation of H3 inhibits Ser-2 phosphorylation by RPS6KA5/MSK1. Phosphorylation at Thr-121 (H2AT120ph) by DCAF1 is present in the regulatory region of many tumor suppresor genes and down-regulates their transcription.Glutamine methylation at Gln-105 (H2AQ104me) by FBL is specifically dedicated to polymerase I. It is present at 35S ribosomal DNA locus and impairs binding of the FACT complex (PubMed:24352239).Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.
Celluar localization	Extracellular region or secreted;Nucleus;
UniPort	P04908