Rabbit Anti-Nanog Polyclonal Antibody#abs131129

abs131129-50ug

1-2 Weeks

201

abs131129-100ug

1-2 Weeks

301

Description

This molecule plays a crucial role in regulating the proliferation and self-renewal of inner cell mass and embryonic stem cells. It is responsible for maintaining the pluripotency of these cells and preventing their differentiation towards extraembryonic endoderm and trophectoderm lineages. By physically interacting with SMAD1, it interferes with the recruitment of coactivators to active SMAD transcriptional complexes, thus blocking bone morphogenetic protein-induced mesoderm differentiation of ES cells. Additionally, it acts as a transcriptional activator or repressor, binding optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, it promotes cells to enter into S phase and increases proliferation.

Other names

Nanog, also known as Embryonic stem cell specific homeobox protein, ENK, FLJ12581, hNanog, or Homeobox protein NANOG, is a crucial transcription factor involved in embryonic development and stem cell maintenance. It plays a significant role in maintaining pluripotency and self-renewal capacities in embryonic stem cells.
The homeobox transcription factor Nanog-delta 48, or simply Nanog, is an essential regulator of gene expression. Its presence ensures the undifferentiated state of embryonic stem cells. Through its binding to DNA, Nanog controls the transcription of numerous genes involved in cellular processes.
Identified as NANOG, this homeobox protein Nanog is responsible for the regulation of embryonic stem cell pluripotency. It assists in maintaining the undifferentiated state of these cells. NANOG has gained recognition for its crucial role in embryonic development and its potential for therapeutic applications.
In summary, NANOG, or Nanog homeobox, is a homeobox transcription factor that plays a critical role in maintaining the pluripotency and self-renewal capacities of embryonic stem cells. Its presence is essential for the regulation of gene expression, ensuring the undifferentiated state of these cells. With its significance in embryonic development, NANOG holds promising potential for future therapeutic advancements.

Source

Rabbit

Specificity

The detection of endogenous levels of total Nanog protein is carried out by the antibody. Let me generate a highly similar content while rearranging the original text:
Utilizing the antibody, we are able to detect the total Nanog protein at its natural levels within an organism.

Application

WB1:500-1:2000，ELISA（）1:20000-1:40000。，。

Nano g，aa.137~141（K-Q-V-K-T），，。，。
"- K-Q-V-K-T- - g- - - aa.- 137~141，- - - - - - - - - - "

Properties

1mg/ml

Purification

The SulfoLink™ Coupling Resin was utilized for peptide affinity chromatography in order to purify the antiserum. This resulted in a highly refined product that is very similar to the original substance.

Clonality

Polyclonal Antibody

To ensure optimal storage conditions, it is recommended that the product be stored at a temperature of -20 °C for a period of one year. It is important to avoid exposing the item to repeated freeze/thaw cycles, as this can cause damage to the product and compromise its quality. By adhering to these guidelines, you can help to ensure that the product remains stable and effective for its intended use over an extended period of time.

Storage buffer

The storage condition for Rabbit IgG includes being stored at -20°C in phosphate-buffered saline with a pH of 7.4. The solution also contains 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is important to note that the stability of the IgG is guaranteed for a period of 12 months from the date of receipt. Please ensure proper storage and handling to maintain the integrity of the product.

Target

Background

 Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter (PubMed:25825768). Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.

Tissue specificity

Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.

Celluar localization

Q9H9S0