Rabbit Anti-SOX2 Polyclonal Antibody#abs131128

abs131128-50ug

1-2 Weeks

201

abs131128-100ug

1-2 Weeks

301

Description

The trimeric complex formed by OCT4 and a transcription factor regulates the expression of various genes essential for embryonic development, including YES1, FGF4, UTF1, and ZFP206. This crucial complex plays a significant role in early embryogenesis and the maintenance of embryonic stem cell pluripotency.

Other names

Sox2, also known as Sex determining region Y box 2 or SRY box containing gene 2, is a transcription factor that plays a crucial role in determining the sex of an individual. It belongs to the SRY-related HMG box gene family and is encoded by the SOX2 gene. The protein encoded by this gene, SOX2_HUMAN, is involved in the regulation of embryonic development and the maintenance of pluripotency in stem cells. Sox2 is a key player in various biological processes and has been extensively studied in both human and animal models. Its importance in development and disease makes it an intriguing target for further research.

Source

Rabbit

Specificity

This antibody is designed to specifically recognize the total SOX2 protein present within the body at its normal levels.

Application

The suggested dilution range for using the Western Blot technique is between 1:500-1:2000. On the other hand, when using ELISA with a peptide, the optimal dilution range is 1:20000-1:40000. It is important to ensure that the dilution is accurate in order to achieve accurate results.

SOX2aa.76~80G-A-E-W-K。，，。

Properties

1mg/ml

Purification

Using SulfoLink™ Coupling Resin, the purification of the antiserum was achieved through peptide affinity chromatography.

Clonality

Polyclonal Antibody

To ensure the longevity of the product, it is recommended to store it at -20 °C for up to one year. It is crucial to avoid exposing the product to repeated freeze/thaw cycles as it may compromise the quality. As such, it is essential to adhere to the recommended storage conditions to maintain the effectiveness of the product.

Storage buffer

This solution contains Rabbit IgG in a buffer of phosphate saline at a pH of 7.4 with 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended to store it at a temperature of -20°C and it is stable for a duration of 12 months from the time of receipt.

Target

Background

This trimeric complex transcription factor, when bound to OCT4 on DNA, plays a crucial role in regulating the expression of several genes involved in embryonic development, including YES1, FGF4, UTF1, and ZFP206 (similar in function). Its importance lies in its involvement in early embryogenesis and maintenance of embryonic stem cell pluripotency. Furthermore, it functions as a key player in neuronal development, acting as a switch in this process. Similar to its role in embryogenesis, it acts as a downstream target of SRRT and facilitates the promotion of self-renewal in neural stem cells (similar in function). This factor also plays a crucial role in maintaining neural cells in an undifferentiated state by counteracting the effects of proneural proteins and suppressing neuronal differentiation (similar in function).

Celluar localization

Cytosol;Nucleus;

P48431