Rabbit Anti-Twist1 Polyclonal Antibody#abs131127

abs131127-50ug

In Stock

201

abs131127-100ug

In Stock

301

Description

This protein functions as a transcriptional regulator and exerts its effects through various mechanisms. It acts by sequestering E proteins, blocking trans-activation by MEF2, and inhibiting the binding of MYOD1 to DNA through physical interaction. The interaction between this protein and its targets likely involves the basic domains present in both proteins. Furthermore, it plays a role in repressing the expression of proinflammatory cytokines like TNFA and IL1B. Its involvement in regulating cranial suture patterning and fusion has also been demonstrated.
When it forms a heterodimer with E proteins, it promotes transcriptional activation. However, its effect on gene expression is different based on the composition of the dimer. Homodimers induce the expression of FGFR2 and POSTN, while heterodimers repress the expression of FGFR2 and POSTN and induce THBS1 expression. It is worth noting that osteoblast differentiation requires the formation of heterodimers. By rearranging the information above, we can generate a similar content while ensuring it remains faithful to the original text.

Other names

Twist-related protein 1, also known as TWIST1 or bHLHa38, is a DNA binding protein belonging to the Class A basic helix-loop-helix (bHLH) family. It is associated with various genetic disorders like BPES2, BPES3, CRS, CRS1, CSO, and SCS. TWIST1 is coded by the OTTHUMP00000116043 gene and is a homolog of the Twist protein found in Drosophila. This transcription factor, also referred to as H-twist, plays a crucial role in regulating gene expression and development. Its functions extend to embryogenesis, craniofacial morphogenesis, cell differentiation, and cancer progression. By rearranging the above information, this glimpse into the TWIST1 protein sheds light on its critical role as a mammalian bHLH transcription factor and its association with several genetic disorders.

Source

Rabbit

Specificity

The total Twist1 protein can be detected by the antibody at endogenous levels. A similar statement can be created by rearranging the original text information.

Application

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，：Western Blotting (WB) 1:500-1:2000； (IHC) 1:50-1:200；/ (IF/ICC) 1:100-1:500； (ELISA) 1:20000-1:40000，。，。

Twist1aa.18~22（S-N-S-E-E）。，，。

Properties

1mg/ml

Purification

Peptide affinity chromatography utilizing SulfoLink™ Coupling Resin was employed for the purification of the antiserum.

Clonality

Polyclonal Antibody

Make sure to store the product at -20 °C for a duration of one year without exposing it to repeated freeze/thaw cycles.

Storage buffer

This product contains Rabbit IgG in a buffer solution of phosphate saline with a pH of 7.4. With a concentration of 150mM NaCl and a small amount of 0.02% sodium azide, this solution is maintained at a liquid state with 50% glycerol. This product should be stored at a temperature of -20 °C to extend its shelf life which is stable for up to 12 months from the date of purchase.

Target

Background

 Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation. Represses the activity of the circadian transcriptional activator: NPAS2-ARNTL/BMAL1 heterodimer (By similarity).

Tissue specificity

Subset of mesodermal cells.

Celluar localization

Q15672