Rabbit Anti-CDK4 Polyclonal Antibody#abs131131

abs131131-50ug

1-2 Weeks

201

abs131131-100ug

1-2 Weeks

301

Description

The activity of cyclin-dependent kinases, such as CDK4, is tightly regulated by several factors. One important mechanism involves the phosphorylation of a specific residue (Thr172) in the CDK's T-loop. In addition, the abundance of cyclin partners and the presence of CDK inhibitors also modulate the kinase's activity.
An interesting feature of CDK4/cyclin D complexes is their ability to integrate extracellular signals to promote cell cycle progression. For instance, the inactive complex formed by CDK4/cyclin D and the CDK inhibitor p27 Kip1/Cip1 requires the presence of mitogenic stimuli to release and degrade p27. This step is crucial for the cells to pass the so-called restriction point and initiate S-phase. Once activated, CDK4/cyclin D complexes phosphorylate retinoblastoma protein, thus triggering the release of E2F transcription factors and allowing the expression of G1/S-phase genes.
Recent studies have uncovered new links between CDK4/cyclin D activity and other cell cycle checkpoints. For instance, in HeLa cells, exposure to UV radiation can upregulate the association between CDK4/cyclin D3 and the CDK inhibitor p16 INK4A, leading to a delay in the G2-phase of the cell cycle. This finding highlights the importance of CDK4/cyclin D3 activity in the regulation of the G2 checkpoint and further underscores the complexity of cell cycle control.

Other names

The CDK4 protein, also known as cell division kinase 4 or p34 cdk4, is a cyclin-dependent kinase that plays a critical role in cell division. It is encoded by the CDK4 gene and is involved in regulating the progression of the cell cycle. CDK4 interacts with cyclin D to form an active complex that phosphorylates key substrates and drives the cell cycle forward. In addition, mutations in the CDK4 gene have been associated with some types of cancer, including melanoma. The protein is also known as melanoma cutaneous malignant 3 or CMM3. Other names for CDK4 include cell division protein kinase 4, Crk3, and PSK-J3. MGC14458 is another name for the CDK4 gene. Overall, CDK4 is an important protein with many functions in normal cell growth and division, as well as in the development of certain types of cancer.

Source

Rabbit

Specificity

The total CDK4 protein in endogenous levels can be detected by this antibody. To convey the same message, the content can be rephrased as follows: This antibody is capable of identifying the entire CDK4 protein present in the endogenous levels.

Application

：WB 1:500-1:2000，ELISA（）1:20000-1:40000。
：
ELISA（），1:20000-1:40000。Western blotting，1:500-1:2000。。。

The amino acid sequence of human CDK4 around residues 295-299 is LHKDE. It is important to note that any alteration in this sequence may significantly impact the function of the protein. Therefore, caution needs to be exercised during any manipulation of this region. It is also worth mentioning that CDK4, in conjunction with other proteins, plays a crucial role in the regulation of the cell cycle and any dysfunction can lead to cell proliferation and tumorigenesis.

Properties

1mg/ml

Purification

Using SulfoLink™ Coupling Resin, the purification of the antiserum was accomplished through peptide affinity chromatography. To create similarly articulated content, we can say that the antiserum underwent a process of purification through the utilization of SulfoLink™ Coupling Resin in peptide affinity chromatography.

Clonality

Polyclonal Antibody

To preserve the quality, store the item at a temperature of -20 °C for a period of one year. It is crucial to avoid subjecting it to multiple cycles of freezing and thawing.

Storage buffer

The storage conditions for Rabbit IgG include maintaining it in a solution of phosphate buffered saline with a pH of 7.4. It should also contain 150mM NaCl, 0.02% sodium azide, and 50% glycerol. To ensure its stability, it should be stored at a temperature of -20 °C. It is important to note that Rabbit IgG is stable for a period of 12 months starting from the date it was received.

Target

Background

 Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.

Celluar localization

Cytosol;Nucleus;

P11802