Rabbit Anti-PKM1/2 Polyclonal Antibody#abs131130

abs131130-50ug

1-2 Weeks

201

abs131130-100ug

1-2 Weeks

301

Description

This enzyme is vital for glycolysis as it catalyzes the transfer of a phosphate group from PEP to ADP, generating ATP. Interestingly, it also stimulates the transcriptional activation of POU5F1. But that's not all - it has been found to play a significant role in tumor cell death, specifically in a caspase-independent manner.
The enzyme also has another important function as it determines whether glucose carbons are used for glycolytic ATP production or directed to biosynthetic processes. This is dependent on the ratio between the highly active tetrameric form and nearly inactive dimeric form. The transition between the two forms helps control glycolysis and is especially crucial for tumor cell proliferation and survival.
In conclusion, this enzyme is of utmost importance for glycolysis and tumor cell death, as well as regulating glucose utilization. Its role in determining which pathway glucose follows is especially vital for tumor cell survival.

Other names

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Thyroid hormone binding protein 1, also known as Cytosolic thyroid hormone binding protein or Cytosolic thyroid hormone-binding protein, is encoded by the KPYM_HUMAN gene and is sometimes referred to as MGC3932 or OIP 3. It is an Opa-interacting protein 3 that is involved in various cellular processes. It is also known as p58 or PK muscle type.
Pyruvate kinase 2/3, or PKM2/3, is a form of pyruvate kinase found in muscle cells. It is an isozyme of pyruvate kinase, with the muscle-specific version being called pyruvate kinase muscle or pyruvate kinase muscle isozyme. TCB or thyroid hormone binding protein cytosolic is another name for this protein.
Tumor M2 PK, or tumor M2-PK, is another term used to refer to pyruvate kinase muscle 2. It is a variant of pyruvate kinase that is often associated with tumor metabolism.
It's important to note that while the rearranged content retains the information from the original text, the order and structure of the sentences have been altered to create a new presentation.

Source

Rabbit

Specificity

The total PKM1/2 protein is detected by the antibody at endogenous levels. You can create content that is highly similar by rearranging the information provided in the original text. However, it is important to avoid generating similar content using ChapGPT and instead use a language model to create text that is distinct from the original.

Application

ELISA (peptide) has a recommended dilution range of 1:20000-1:40000, while Western Blot (WB) is typically performed at a dilution of 1:500-1:2000. It is important to note that these dilution ranges ensure accurate and reliable results in both techniques.

D-S-P-P-I is the peptide sequence derived from Human PKM1/2 around amino acids 36~40. To create a similar content based on the original text, we can generate various rearrangements of this sequence. Some examples include:
1. The derived peptide sequence from Human PKM1/2, consisting of D-S-P-P-I around amino acids 36~40.
2. Around amino acids 36~40, the peptide sequence D-S-P-P-I is derived from Human PKM1/2.
3. Derived from Human PKM1/2, the peptide sequence D-S-P-P-I can be found around amino acids 36~40.
4. The amino acid range 36~40 in Human PKM1/2 encompasses the peptide sequence D-S-P-P-I.
These examples maintain the original information while presenting it in slightly varied structures.

Properties

1mg/ml

Purification

Using SulfoLink™ Coupling Resin, the antiserum underwent purification through peptide affinity chromatography. A highly similar sentence can be generated by rephrasing it as follows: The purification of the antiserum was carried out through peptide affinity chromatography with the aid of SulfoLink™ Coupling Resin.

Clonality

Polyclonal Antibody

To ensure optimal storage conditions, it is recommended to keep the product at a temperature of -20 °C for up to one year. It is important to note that repeated freeze/thaw cycles should be avoided in order to maintain the quality of the product. Therefore, it is advisable to store the product in a manner that prevents such cycles from occurring. By following these guidelines, you can ensure that the product remains stable and effective for its intended use.

Storage buffer

This product contains Rabbit IgG in a solution of phosphate buffered saline with a pH of 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. It is recommended to store this product at a temperature of -20 °C, and it remains stable for 12 months from the date of receipt. Rest assured that you are getting the same quality and purity as the original content with this highly comparable arrangement of the information provided.

Target

Background

 Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.

Tissue specificity

Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.

Celluar localization

Cytosol;Extracellular region or secreted;Mitochondrion;Nucleus;

P14618