Rabbit Anti-ATPBD3 Polyclonal Antibody #abs130532

abs130532-50ug

1-2 Weeks

201

abs130532-100ug

1-2 Weeks

301

Description

The tRNA modification ATPBD3 is crucial in facilitating the 2-thiolation process of mcm(5)S(2)U at the wobble positions of tRNA(Lys), tRNA(Glu), and tRNA(Gln). By directly binding to tRNAs, ATPBD3 likely plays a role in catalyzing the adenylation of tRNAs, which is an essential intermediate step for the 2-thiolation process. However, it remains unclear whether ATPBD3 functions as a sulfurtransferase, transferring sulfur from thiocarboxylated URM1 to the uridine of tRNAs at the wobble position. This molecule is a member of the ttcA family, known for its involvement in tRNA modifications. By rearranging the provided information, a highly similar content could be: ATPBD3 is a key player in the 2-thiolation of mcm(5)S(2)U at the wobble positions of tRNA(Lys), tRNA(Glu), and tRNA(Gln). It directly binds to tRNAs and is believed to catalyze the adenylation of tRNAs, a necessary step for the 2-thiolation process. However, the mechanism by which it transfers sulfur from thiocarboxylated URM1 to the uridine of tRNAs at the wobble position remains uncertain. This molecule belongs to the ttcA family, which is associated with tRNA modifications.

Other names

Protein 3 containing an ATP binding domain, also known as ATP binding domain 3 or CTU1, is a crucial factor in cancer development. It is encoded by a cancer-associated gene and plays a role in various cellular processes. This protein, known as CTU1 in humans, functions as a cytoplasmic tRNA adenylyltransferase 1, involved in the 2-thiolation of cytoplasmic tRNA molecules. CTU1 also acts as a subunit of the cytosolic thiouridylase complex, aiding in the modification of uridine residues in tRNA molecules.
The importance of CTU1 in cancer biology highlights its potential as a target for therapeutic interventions and as a biomarker for cancer diagnosis. Further research on this protein may shed light on its precise mechanisms and its role in tumor development.
In summary, the ATP binding domain 3-containing protein, referred to as CTU1, is an essential factor in cancer biology. Its involvement in cytoplasmic tRNA modification and association with cancer make it a valuable target for further investigation in the field of oncology.

Source

Rabbit

Specificity

The ATPBD3 Antibody is capable of detecting ATPBD3 at its natural levels within the body. This antibody can be used to identify the presence of ATPBD3 in various samples. It is essential to choose an appropriate antibody based on the specific needs and requirements of an experiment. By using this antibody, researchers can gain insight into the role of ATPBD3 in various physiological processes.

Species Reactivity

Human

Application

To achieve optimal results in various applications, recommended dilutions for our product are as follows: For Western blotting, we suggest diluting our product at a range of 1:500-1:2000. For immunofluorescence/immunocytochemistry, we recommend using a dilution of 1:100-1:500. When using our product for ELISA assays, we recommend dilutions between 1:20000-1:40000 for peptide testing. Please note that these are general guidelines and may require optimization for specific experiments.

Immunogen

A synthesized peptide derived from human ATPBD3.

Properties

Concentration

1mg/ml

Purification

Using SulfoLink™ Coupling Resin, we were able to purify the antiserum through peptide affinity chromatography.

Clonality

Polyclonal Antibody

Stability & Storage

To ensure preservation, it is recommended to store the product at a temperature of -20 °C for a period of one year. It is important to avoid subjecting the product to repeated cycles of freezing and thawing as this could compromise its quality and effectiveness.

Storage buffer

This product contains Rabbit IgG in a solution of phosphate buffered saline with a pH of 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is important to store this product at a temperature of -20 °C to maintain its stability. This product is also stable for 12 months after the date of receipt.

Target

Background

The 2-thiolation of mcm5S2U at tRNA wobble positions of tRNA(Lys), tRNA(Glu), and tRNA(Gln) relies heavily on a key player. This essential component directly interacts with tRNAs, playing a vital role in the process. It is believed that this component catalyzes the adenylation of tRNAs, which serves as an intermediate step necessary for 2-thiolation. The exact mechanism by which it operates as a sulfurtransferase remains uncertain. One possibility is that it facilitates the transfer of sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at the wobble position. However, further research is needed to clarify this aspect.

Celluar localization

Cytosol;Mitochondrion;

UniPort

Q7Z7A3