Phospho-Ezrin (Thr566) Rabbit Polyclonal Antibody#abs130799

abs130799-50ug

1-2 Weeks

201

abs130799-100ug

1-2 Weeks

301

Description

This gene encodes a cytoplasmic peripheral membrane protein that is vital for microvilli function. It acts as a substrate for protein-tyrosine kinase in microvilli and belongs to the ERM protein family. What sets this protein apart is its role as an intermediary between the plasma membrane and the actin cytoskeleton. Its ability to connect the two enables proper cellular structure and function.

Other names

The human protein Ezrin, also known as Villin 2 or Cytovillin 2, plays a crucial role in cytoskeletal organization and cell signaling. It is encoded by the EZR gene and is expressed in a wide range of human tissues, including the epithelial cells lining the organs of the digestive, respiratory, and urogenital systems.
Ezrin belongs to the ERM protein family, which also includes Radixin and Moesin. These proteins are involved in linking the plasma membrane to the cytoskeleton and in regulating cell shape, adhesion, and motility.
Ezrin has been implicated in various physiological and pathological processes, such as cell migration, tumor invasion and metastasis, inflammation, and infection by viruses and bacteria. It interacts with a multitude of cellular and extracellular proteins, including receptors, kinases, phosphatases, adhesion molecules, and cytoskeletal components.
Mutations or dysregulation of Ezrin have been associated with several human diseases, such as cancer, autoimmune disorders, and viral infections. Therefore, Ezrin is a potential target for diagnostic, prognostic, and therapeutic purposes in medicine.

Source

Rabbit

Specificity

The Phospho-Ezrin (Thr566) Antibody has the ability to specifically detect the presence of the endogenous levels of Ezrin protein, but only when it is phosphorylated on Threonine 566. This antibody specifically targets the phosphorylated form of Ezrin, and is not capable of detecting the non-phosphorylated form of the protein. The raised antibody binds specifically to the phosphorylated threonine residue on the protein, allowing it to be uniformly detected and measured in biological samples. The unique specificity of this antibody makes it an essential tool for researchers studying the regulation and function of Ezrin protein in various cellular processes.

Predictive reaction species

Xenopus;Chicken;Rabbit;Pig;Dog;Horse;Bovine

Application

。：
- WB: 1:500-1:2000
- IF/ICC: 1:100-1:500
- ELISA（）: 1:20000-1:40000
。

A peptide that has been synthesized from human Ezrin and is linked to the phosphorylation site of Threonine 566 is available for use. This peptide closely resembles the original sequence and can be used for a variety of research applications. The careful selection of amino acids ensures high specificity in binding and enhanced stability, making it a reliable tool for researchers. It can be used in several different assays to study the effects of phosphorylation on Ezrin and other related proteins.

Properties

1mg/ml

Purification

Sequential chromatography was used to purify the antibody from rabbit serum. This process involved affinity purification on both phospho- and non-phospho-peptide columns. As a result, the antibody is highly purified and optimized for its intended use.

Clonality

Polyclonal Antibody

To ensure optimal storage, it is recommended that the product be kept at a temperature of -20 °C for a period of one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles in order to maintain its effectiveness.

Storage buffer

Our Rabbit IgG is stored in a solution of phosphate buffered saline with a pH of 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol. To maintain its stability, it should be stored at -20 °C. You can expect this product to remain stable for 12 months from the date of receipt.

Target

Background

It is believed that this protein plays a crucial role in linking major cytoskeletal structures to the plasma membrane. Specifically, in epithelial cells, this protein is required for the formation of microvilli and membrane ruffles on the apical pole. Additionally, along with the protein PLEKHG6, it is essential for proper macropinocytosis.

Tissue specificity

Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied.

Celluar localization

Cytoskeleton;Cytosol;Endosome;Extracellular region or secreted;Nucleus;Plasma Membrane;

P15311