Rabbit Anti-ANG2 Polyclonal Antibody#abs131201

abs131201-50ug

1-2 Weeks

201

abs131201-100ug

1-2 Weeks

301

Description

TIE2 can be induced to undergo tyrosine phosphorylation by ANG2. When ANG2 binds to the TIE2 receptor, it interferes with angiopoietin-1's ability to promote blood vessel maturation and stability. However, the effects of ANG2 may depend on the specific context. For example, in the absence of angiogenic inducers like VEGF, ANG2 might cause endothelial cells to detach from the extracellular matrix, leading to cell death and ultimately the regression of blood vessels.

Other names

ANGPT2, also known as AGPT 2, ANG-2, ANG2 or Angiopoietin-2, is a protein that acts as a ligand for the Tie2 receptor. It is involved in the regulation of blood vessel formation and maintenance, as well as in inflammation and tissue repair processes. ANGPT2 is expressed in various cell types, including endothelial cells, smooth muscle cells, and pericytes, and its expression is modulated by various physiological and pathological factors. ANGPT2 is a potential therapeutic target for various diseases, including cancer, cardiovascular disease, and sepsis.

Source

Rabbit

Specificity

The ANG 2 Antibody is capable of detecting total ANG 2 levels in the body that are naturally present. This means that the antibody is sensitive to the native form of ANG 2, making it a reliable tool for many research applications. By targeting endogenous ANG 2, the antibody is able to provide accurate results that can be used to further our understanding of various biological processes. Overall, the ANG 2 Antibody is a highly effective tool for studying ANG 2 and its functions in the body.

Application

For Western Blotting, the recommended dilution range is 1:500-1:2000. As for Immunohistochemistry, the optimal dilution range is 1:50-1:200. When it comes to ELISA using a peptide as the target antigen, a dilution range of 1:20000-1:40000 is the recommended range. It is important to ensure that the dilution is appropriate in order to obtain accurate and reliable results.

A synthesized peptide derived from human ANG 2.

Properties

1mg/ml

Purification

Using SulfoLink™ Coupling Resin, peptide affinity chromatography was employed in purifying the antiserum. To ensure the highest quality outcome, this was done through a meticulous process. Through this process, the antiserum was effectively purified, achieving optimal results.

Clonality

Polyclonal Antibody

To maintain the quality of the product, it is advised to store it at a temperature of -20°C for a duration of one year. It is recommended to avoid exposing it to repeated cycles of freezing and thawing.

Storage buffer

The rabbit IgG is stored in a solution consisting of phosphate buffered saline with a pH of 7.4, 150 mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be stored at a temperature of -20 °C. The stability of the IgG is guaranteed for a period of 12 months starting from the date of receipt.

Target

Background

ANGPT2 is a protein that binds to TEK/TIE2, competing with ANGPT1 for its binding site. This competition modulates the signaling of ANGPT1. Interestingly, ANGPT2 can induce the phosphorylation of TEK/TIE2 even in the absence of ANGPT1.
When there is a lack of angiogenic inducers like VEGF, ANGPT2 can cause the loosening of cell-matrix contacts, which may lead to apoptosis of endothelial cells and subsequent vascular regression.
However, when VEGF is present, ANGPT2 can work together with it to promote endothelial cell migration and proliferation. In this way, ANGPT2 acts as a permissive signal for angiogenesis.
To summarize, ANGPT2 plays a complex role in angiogenesis by modulating TEK/TIE2 signaling, inducing cell apoptosis in the absence of angiogenic inducers, and promoting cell migration and proliferation in the presence of VEGF.

Celluar localization

O15123