Rabbit Anti-NAB2 Polyclonal Antibody #abs130442

abs130442-50ug

1-2 Weeks

201

abs130442-100ug

1-2 Weeks

301

Description

The zinc finger transcription factors EGR1 and EGR2 are known to be repressed by NAB2, which acts as a transcriptional repressor. However, it should be noted that the isoform 2 does not possess this repression ability as the first isoform. This information can help us better understand the roles that NAB2 plays in regulating gene expression and protein synthesis. The ability of NAB2 to selectively silence the expression of these important transcription factors highlights the intricate complexity of the regulatory mechanisms that govern cellular processes.

Other names

EGR1 binding protein 2, also known as EGR-1-binding protein 2 or MADER, is a protein that is associated with melanoma and delayed early response. It is also referred to as Melanoma-associated delayed early response protein or NGFI A binding protein 2. Other names for this protein include MGC75085 and Nab 2. The human gene encoding this protein is called NAB2. It plays a role in binding to the NGFI-A gene. Another name for this protein is Protein MADER.

Source

Rabbit

Specificity

The EGR1/2 Antibody is capable of detecting the naturally occurring levels of EGR1/2 within cells. By rearranging the original content, a highly similar statement can be made without changing the context of the information provided.

Species Reactivity

Human;Mouse

Application

For immunohistochemistry (IHC), use a dilution range of 1:50 to 1:200 and for western blot (WB), a dilution range of 1:500 to 1:2000. ELISA using peptide should be diluted from 1:20000 to 1:40000. These are the recommended dilution ranges for optimal results in these assays.

Immunogen

A synthesized peptide derived from human EGR1/2

Properties

Concentration

1mg/ml

Purification

Immunogen affinity purified

Clonality

Polyclonal Antibody

Stability & Storage

To maintain optimal storage conditions, it is recommended to store the product at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles.

Storage buffer

-20°C-。IgG150mM NaCl，pH7.4 saline，0.02%50%。

Target

Background

EGR1 and EGR2 are zinc finger transcription factors that can be regulated by a transcriptional repressor. A specific isoform, isoform 2, lacks the ability to repress these factors. This indicates that the isoform 2 does not possess the same inhibitory function as the original transcriptional repressor.

Tissue specificity

Melanoma cell lines display high expression levels compared to the low expression levels found in other tissues where it is also widely expressed.

Posttranslational modification

EGR2, through its process of sumoylation, has been discovered to have a repressive effect on its own transcriptional activity within the hindbrain. This finding underscores the complexity of regulatory mechanisms involved in the function of EGR2, and highlights the importance of understanding the interplay between post-translational modifications and gene expression in neurological processes. Further research is needed in order to fully elucidate the mechanisms behind EGR2's self-regulation and the implications of this discovery for future therapeutic interventions.

Celluar localization

Nucleus;

UniPort

Q15742