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Rabbit Anti-NSE Polyclonal Antibody #abs130159

Description

Catalog-specification	Delivery time	USD price
abs130159-50ug	1-2 Weeks	201
abs130159-100ug	1-2 Weeks	301

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Overview
Description	EN02 is an important enzyme that exhibits 2-phospho-D-glycerate hydro-lyase activity. This enzyme is one of the three major enolase isoenzymes found in mammals. Interestingly, this specific isoenzyme is a homodimer and is predominantly expressed in mature neurons and cells of neuronal origin. During development in rats and primates, a significant switch from alpha enolase to gamma enolase occurs in neural tissue. These findings suggest that ENO2 plays an important role in the development of the nervous system and may be involved in regulating the activities of mature neurons.
Other names	Enolase 2, also known as 2-phospho-D-glycerate hydro-lyase or 2 phospho D glycerate hydrolyase, is an important enzyme in the human body. It goes by various names such as Eno 2, ENO2, ENOG, ENOG_HUMAN, and Enolase 2 gamma neuronal. This enzyme plays a crucial role in glycolysis, the metabolic pathway that converts glucose into energy. Enolase 2 is specifically expressed in neurons and is commonly referred to as neuron-specific enolase (NSE). It is also found in the epididymis, where it is known as epididymis secretory protein Li 279 (HEL S 279). This enzyme has been extensively studied due to its association with neurological disorders and diseases. Gamma enolase, as it is sometimes called, is involved in the catalysis of the conversion of 2-phospho-D-glycerate into phosphoenolpyruvate, a key step in glycolysis. It is essential for the production of ATP, the energy currency of cells. The levels of neuron-specific enolase in the bloodstream can serve as a diagnostic marker for certain neurological conditions and tumors. Overall, enolase 2 is a crucial enzyme involved in energy production and regulation in neurons and other tissues. Its unique expression pattern and functional significance make it a subject of great interest in both basic research and clinical applications. NSE is essential for maintaining proper neuronal function and its dysregulation has been implicated in various diseases and disorders.
Source	Rabbit
Specificity	The NSE antibody has the ability to identify the total NSE present in an organism naturally. This means that it can detect the existing levels of NSE without the need for any external simulation or intervention.
Species Reactivity	Human;Mouse;Rat
Application	The recommended dilutions for Western blotting (WB) range from 1:500 to 1:3000. For immunohistochemistry (IHC), the suggested dilutions are between 1:50 and 1:200. For immunofluorescence/immunocytochemistry (IF/ICC), dilutions of 1:100 to 1:500 are typically used. In the case of ELISA using peptides, the dilutions range from 1:20000 to 1:40000. These dilutions serve as guidelines and may need to be optimized for specific experimental conditions.
Immunogen	A synthesized peptide derived from human NSE.
Properties
Concentration	1mg/ml
Purification	SulfoLink™ Coupling Resin was utilized for peptide affinity chromatography to purify the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain the quality of the product, it is recommended to store it at a temperature of -20 °C for a period of one year. It is important to avoid subjecting the product to frequent freeze and thaw cycles.
Storage buffer	This solution contains Rabbit IgG in a buffer of phosphate saline, pH 7.4, with 150mM NaCl and 0.02% sodium azide. To prevent degradation, it is recommended to store at -20 °C with 50% glycerol added. This solution has a shelf life of 12 months from the date of receipt.
Target
Background	This substance displays both neurotrophic and neuroprotective characteristics towards a wide variety of neurons found in the central nervous system (CNS). It binds to neocortical neurons, promoting cell survival in a calcium-dependent manner, as observed in laboratory studies.
Tissue specificity	The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
Celluar localization	Cytosol;Extracellular region or secreted;Plasma Membrane;
UniPort	P09104