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Rabbit Anti-NM23 Polyclonal Antibody #abs130158

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Description

Catalog-specification	Delivery time	USD price
abs130158-50ug	1-2 Weeks	201
abs130158-100ug	1-2 Weeks	301

Overview
Description	Nucleoside diphosphate kinase A, also known as NM23, plays a vital role in the synthesis of nucleoside triphosphates, which are essential for various cellular processes. The protein is composed of hexamer holoenzyme, consisting of both A and B subunits, and mutations in the gene have been linked to aggressive neuroblastomas. There are two transcript variants of NM23, which lead to the formation of different isoforms. Both isoforms are involved in catalyzing the transfer of phosphate groups between nucleoside molecules, thus playing a pivotal role in DNA replication and repair. Despite being originally discovered as a metastasis-suppressing protein, NM23 has been found to have diverse functions in different cellular pathways, including signal transduction and cytoskeleton organization. Its significance in various physiological processes makes it an important target for therapeutic interventions against cancer and other diseases.
Other names	Possible highly similar content: The human gene known as c-myc encodes a transcription factor that binds to purine-rich DNA sequences. This protein is also called PUF and plays a crucial role in regulating cell proliferation, differentiation, and apoptosis. C-myc is expressed in many tissues and is involved in various physiological and pathological processes, including cancer. Another name for c-myc is NME2, which stands for non-metastatic cells 2, a protein that belongs to the nucleoside diphosphate kinase (NDPK) family. NME2 is also known as NM23-H2 or NDPK-B and has been shown to have multiple functions in addition to its enzymatic activity, such as interacting with signaling molecules and affecting chromatin structure. Mutations or overexpression of c-myc or NME2 have been associated with various diseases, particularly cancer. The protein encoded by c-myc/NME2 has been extensively studied and is a target for therapeutic interventions.
Source	Rabbit
Specificity	Total NM23 can be detected with the NM23 antibody at endogenous levels. To create similar content, the information can be rearranged to say that the NM23 antibody is capable of detecting the entirety of NM23 that exists naturally within an organism.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Rabbit;Pig;Dog
Application	The dilution ratios for Western Blotting (WB) range from 1:500 to 1:3000. For Immunofluorescence/Immunocytochemistry (IF/ICC), the recommended dilution ratios are 1:100 to 1:500. When conducting ELISA with peptide detection, it is advised to use dilution ratios of 1:20000 to 1:40000. Please note that this generated content is similar to the original text but rearranged to provide a different perspective.
Immunogen	A synthesized peptide derived from human NM23.
Properties
Concentration	1mg/ml
Purification	Using SulfoLink™ Coupling Resin, the antiserum was subjected to peptide affinity chromatography for purification. To obtain a highly purified antiserum, this method was employed.
Clonality	Polyclonal Antibody
Stability & Storage	Please adhere to the following guidelines to ensure the stability and quality of the stored product: store it at a temperature of -20 °C for a period of one year, taking care to avoid any freeze/thaw cycles that may occur. Following these instructions will help maintain the integrity of the stored item.
Storage buffer	This solution contains Rabbit IgG in phosphate buffered saline with a pH of 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be stored at -20 °C and remains stable for 12 months from the date of receipt.
Target
Background	This molecule plays a crucial role in the production of nucleoside triphosphates other than ATP. Moreover, it is involved in the regulation of Rho activity by interacting with AKAP13/LBC. Another function of this molecule is activating the MYC gene as a transcriptional activator. To achieve this, it binds DNA in a non-specific manner. Interestingly, it also exhibits histidine protein kinase activity.
Tissue specificity	Isoform 1 and isoform 3 are ubiquitously expressed.
Celluar localization	Cytoskeleton;Cytosol;Extracellular region or secreted;Mitochondrion;Nucleus;
UniPort	P22392