Rabbit Anti-RAD50 Polyclonal Antibody#abs136696
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Description
| Catalog-specification | Delivery time | USD price |
abs136696-100ug | 1-2 Weeks | 301.0 |
abs136696-50ug | 1-2 Weeks | 201.0 |
Please be advised that the price stated above is only for reference purposes. To obtain the detailed price, kindly get in touch with our seller, Vecent. It is important to note that the information provided is based on the original text, and we encourage you to contact our sales representative for further clarification on the pricing.
| Overview | |
catalog | abs136696 |
Description | The Rad50 protein, which is encoded by this gene, shares remarkable similarities with Saccharomyces cerevisiae Rad50. This specific protein plays a crucial role in repairing DNA double-strand breaks. By forming a complex with MRE11 and NBS1, it exhibits various enzyme activities essential for joining DNA ends through nonhomologous mechanisms. Alongside its partners, this protein assumes a vital function in DNA double-strand break repair, activation of cell cycle checkpoints, maintenance of telomeres, and facilitation of meiotic recombination. Studies involving the knockout of the mouse homolog of this gene have demonstrated its indispensability for cell growth and viability. Notably, mutations in this gene have been identified as the underlying cause of a disorder resembling Nijmegen breakage syndrome. |
| Other names | RAD50 is a DNA repair protein that is also known as hRAD50, hsRAD50, Mrell, NBSLD, Rad 50, RAD50 2, rad50, RAD50 homolog (S cerevisiae), RAD50 homolog, RAD50 PEN, RAD50, S. cerevisiae, homolog of, RAD50_HUMAN, RAD502, Rad50l, or Truncated RAD50 protein. This protein plays a crucial role in maintaining genomic stability by repairing damaged DNA. It is highly similar to its yeast counterpart and exhibits conserved functions in various organisms. RAD50 forms a complex with MRE11 and NBS1 to form the MRN complex, which functions as a sensor of DNA double-strand breaks (DSBs). Upon sensing DNA damage, RAD50 activates a complex network of DNA repair pathways, including homologous recombination and non-homologous end joining, to restore genomic integrity. The RAD50 protein possesses a characteristic structural motif called the ABC-ATPase domain, which is involved in ATP binding and hydrolysis. This domain allows RAD50 to undergo conformational changes necessary for its DNA repair functions. Additionally, RAD50 contains coiled-coil regions that facilitate protein-protein interactions and DNA binding. Defects in RAD50 can lead to genomic instability and predispose individuals to cancer development. Inherited mutations in RAD50 are associated with Nijmegen breakage syndrome, a rare genetic disorder characterized by impaired DNA repair and increased sensitivity to ionizing radiation. Furthermore, dysregulation of RAD50 expression has been observed in various cancers, highlighting its significance in tumor progression. In summary, RAD50 is a crucial DNA repair protein that plays a vital role in maintaining genomic stability. Its interactions with other proteins and its ATPase activity enable efficient repair of DNA damage, ensuring the integrity of the genome. Further research on RAD50 and its functions may provide insights into developing novel strategies for cancer treatment and prevention. |
| Source | Rabbit |
| Specificity | Total RAD50 levels can be detected by the RAD50 Antibody, which specifically targets the endogenous expression of RAD50. By utilizing this antibody, researchers can accurately assess the overall amount of RAD50 protein present in a biological sample. |
| Species Reactivity | Human;Mouse;Rat |
| Antigen | RAD50 |
| Application | The dilution ratios for WB are 1:500 to 1:2000, for IHC are 1:50 to 1:200, for IF are 1:100 to 1:400, and for ELISA (peptide) are 1:20000 to 1:40000. It is important to properly dilute the samples in order to achieve accurate and reliable results in these assays. |
| Immunogen | A synthetic peptide of humanRAD50. |
| MW | 154kDa |
| Properties | |
Concentration | 1mg/ml |
| purification | Using SulfoLink™ Coupling Resin, the antiserum underwent peptide affinity chromatography purification. A highly similar content can be generated by rearranging the sentence structure and using synonymous words while retaining the original meaning. |
| Clonality | Polyclonal Antibody |
| Stability & Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles |
| Storage buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. |
Target | |
Background | Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11 to prevent nucleolytic degradation past a given point (PubMed:11741547, PubMed:9590181, PubMed:9705271, PubMed:9651580). The complex may also be required for DNA damage signaling via activation of the ATM kinase (PubMed:15064416). In telomeres the MRN complex may modulate t-loop formation (PubMed:10888888). |
| Tissue specificity | Expressed at very low level in most tissues, except in testis where it is expressed at higher level. Expressed in fibroblasts. |
| Celluar localization | Nucleus; |
| UniPort | Q92878 |
Western blot analysis of extracts of various celllines, using RAD50antibody|Immunohistochemistryof paraffin-embeddedhuman gastric tissue usingRAD50antibody.
This product is for research use only, not for use in diagnostic prodecures or in human.
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