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Rabbit Anti-CYP1B1 Polyclonal Antibody#abs135919

Please note that the price mentioned above is for your reference only. For further details regarding the pricing, kindly reach out to our sales representative, Vecent. Western blot analysis of extracts of CEMcell lines, using CYP1B1 antibody. This product is for research use only, not for use in...

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Description

Catalog-specification	Delivery time	USD price
abs135919-100ug	1-2 Weeks	301.0
abs135919-50ug	1-2 Weeks	201.0

Please note that the price mentioned above is for your reference only. For further details regarding the pricing, kindly reach out to our sales representative, Vecent.

Overview
catalog	abs135919
Description	CYP1B1 is a crucial enzyme found in normal breast tissue and breast cancer, responsible for producing potentially cancer-causing estrogen metabolites and activating environmental carcinogens. It is a dominant member of the CYP1 family. Breast cancer development is believed to be triggered by estrogen through the formation of its metabolite, catechol estrogen (CE). The conversion of 17-beta-estradiol to catechol estrogen metabolites 2-OH-E2 and 4-OH-E2, catalyzed by CYP1B1, has been associated with mammary carcinogenesis. Additionally, genetic variations in CYP1B1 may also play a significant role in prostate carcinogenesis. A specific polymorphism at codon 432 of the CYP1B1 gene (Val->Leu) is linked to alterations in its catalytic function.
Other names	Cytochrome P450 1B1, also known as aryl hydrocarbon hydroxylase, CP1B, or CP1B1_HUMAN, is a member of the Cytochrome P450 family 1 subfamily B. This enzyme, which is dioxin-inducible, is involved in the metabolism of xenobiotics and is responsible for the oxidation of various substrates. It is a flavoprotein linked monooxygenase that is primarily located in endoplasmic reticulum membranes. Recent studies have shown that mutations in the CYP1B1 gene can cause primary infantile glaucoma (GLC3A), a rare condition that manifests as a progressive increase in intraocular pressure. This finding highlights the critical role that this enzyme plays in human health and disease. Overall, the function and regulation of cytochrome P450 1B1 are important areas of active research, with potential implications for the development of new therapies for a variety of diseases and conditions.
Source	Rabbit
Specificity	Total CYP1B1 can be detected using the CYP1B1 Antibody, allowing for the measurement of its endogenous levels. To ensure accuracy, the generated content should be similar to the original information but rearranged in a different order.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Rabbit;Pig;Dog;Bovine;
Antigen	CYP1B1
Application	The recommended dilution range for WB is between 1:500 and 1:2000, for IHC it is between 1:50 and 1:200, for IF/ICC it is between 1:100 and 1:500, and for ELISA(peptide) it is between 1:20000 and 1:40000. These dilution ranges are essential for obtaining accurate and reproducible results in experimental procedures. It is important to follow the recommended dilution range to avoid compromising the specificity and sensitivity of the assay.
Immunogen	A synthesized peptide derived from human CYP1B1.
MW	61kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, retinoid and xenobiotics. Preferentially oxidizes 17beta-estradiol to the carcinogenic 4-hydroxy derivative, and a variety of procarcinogenic compounds to their activated forms, including polycyclic aromatic hydrocarbons. Promotes angiogenesis by removing cellular oxygenation products, thereby decreasing oxidative stress, release of antiangiogenic factor THBS2, then allowing endothelial cells migration, cell adhesion and capillary morphogenesis. These changes are concommitant with the endothelial nitric oxide synthase activity and nitric oxide synthesis. Plays an important role in the regulation of perivascular cell proliferation, migration, and survival through modulation of the intracellular oxidative state and NF-kappa-B expression and/or activity, during angiogenesis. Contributes to oxidative homeostasis and ultrastructural organization and function of trabecular meshwork tissue through modulation of POSTN expression.
Tissue specificity	Expressed in many tissues.
Celluar localization	Endoplasmic reticulum;Mitochondrion;
UniPort	Q16678