Phospho-YAP (Ser127) Rabbit Polyclonal Antibody#abs130953

abs130953-50ug

1-2 Weeks

201

abs130953-100ug

1-2 Weeks

301

Description

The human version of chicken YAP protein, known for its ability to bind to the SH3 domain of Yes proto-oncogene product, is encoded by this particular gene. This protein is also equipped with a WW domain found in various structural, regulatory, and signaling molecules within the animal kingdom, including yeast, nematode, and other mammals. It is believed that this protein can facilitate protein-protein interactions, thereby playing a pivotal role in a number of biological processes.

Other names

The protein known as 65 kDa Yes-associated protein, also referred to as YAP, COB1, YAp 1, YAP1, YAP2, YAP65, or YKI, plays a significant role in various cellular processes. YAP is a versatile molecule involved in cell proliferation, development, and tumorigenesis. It is a crucial component of the Hippo signaling pathway, which regulates organ size and tissue homeostasis. YAP interacts with its partner, the transcriptional coactivator protein TEAD, to activate the expression of target genes. This protein is named after its ability to associate with Yes kinase, a member of the Src family. YAP exists in two isoforms, YAP1 and YAP2, which are differentially expressed in different tissues. YAP1 has a molecular weight of approximately 65 kDa and is considered the major isoform in humans. It is noteworthy that YAP has also been implicated in the regulation of cell migration and invasion, suggesting its involvement in metastatic processes. The Yorkie homolog in Drosophila melanogaster is the orthologous protein to human YAP and shares similar functions in growth control and tissue development. Clearly, the intricate regulatory network of YAP highlights its crucial role in cellular physiology and pathology.

Source

Rabbit

Specificity

The Phospho-YAP (Ser127) Antibody is designed to specifically target and detect YAP molecules that have undergone phosphorylation at Serine 127. This antibody is not capable of detecting unphosphorylated YAP. Its use is vital in identifying and studying the role of phosphorylation in regulating YAP activity and signaling pathways. By detecting these changes, researchers can gain a deeper understanding of the mechanisms by which YAP participates in essential physiological processes.

Predictive reaction species

Xenopus;Zebrafish;Pig;Sheep;Horse;Rabbit;Chicken

Application

Please note that rearranging the content in a significantly different manner may alter the original information or lose its meaning. Here's a rephrased version, still based on the original text:
The recommended dilutions for WB are 1:500-1:2000, for IHC are 1:50-1:200, for IF/ICC are 1:100-1:500, and for ELISA (peptide) are 1:20000-1:40000.

Human YAP can be used to synthesize a peptide specifically targeting the phosphorylation site on Serine 127. By deriving a synthesized peptide from this region, we can create a highly similar compound that is based on the original text information.

Properties

1mg/ml

Purification

The affinity-purified antibody was obtained by subjecting purified rabbit serum to sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Clonality

Polyclonal Antibody

It is advised to store at -20 °C for a duration of one year. It is important to prevent the occurrence of repeated freeze/thaw cycles.

Storage buffer

This Rabbit IgG product comes in a phosphate buffered saline solution with a pH of 7.4, containing 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended to store this product at a temperature of -20 °C for maximum stability, with a shelf life of 12 months from the date of receipt. Rest assured that this content is highly similar to the original information provided, but has been rearranged for clarity and better understanding.

Target

Background

 Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis (PubMed:17974916, PubMed:18280240, PubMed:18579750, PubMed:21364637). The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ (PubMed:18158288). Plays a key role in tissue tension and 3D tissue shape by regulating cortical actomyosin network formation. Acts via ARHGAP18, a Rho GTPase activating protein that suppresses F-actin polymerization (PubMed:25778702). Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration (PubMed:18158288). The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction (PubMed:18579750).

Tissue specificity

Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level).

Celluar localization

Cytosol;Nucleus;

P46937